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Figure 1: Data from our lab regarding SPIKE stabilized in a detergent mycelle.<br>A: SDS-Page of purified SPIKE in detergent mycelle.<br>B: Western blot of Rho1d4 tagged SPIKE, originated from the SDS Page seen in "A"
Figure 1: Data from our lab regarding SPIKE stabilized in a detergent mycelle.
A: SDS-Page of purified SPIKE in detergent mycelle.
B: Western blot of Rho1d4 tagged SPIKE, originated from the SDS Page seen in "A"

SARS-CoV-2 (COVID-19) Full-Length Beta Variant (B.1.351, SA) Spike Recombinant Protein

CATALOG NUMBER: 21-819

Tested Applications:
ELISA
Specifications
source-species:
HEK293 cells
Species:
SARS-CoV-2 (COVID-19, 2019-nCoV) Spike Protein
source-species:
HEK293 cells
Recombinant Protein Sequence:
del 144, K417N, E484K, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H
furin cleavage site “RRAR” mutated to “GSAG”; KV986PP
C-terminal Rho1D4 tag fused with spacer “GSSG” to protein sequence
Fusion Tag:
C-terminal Rho1D4
Tested Applications:
ELISA
Application Note:
This recombinant protein can be used for ELISA assays, Ligand binding assays (e.g. SPR), Biochemical and biophysical analyses. For research use only.
Predicted Molecular Weight:
1286 amino acids (including Rho1D4 tag and linker); 142114 Da
Biological Activity:
not determined
Purity:
>98% as determined by SDS-PAGE, see Fig. 1 A and B. PureCube Rho1D4 Agarose.
physical-state:
Liquid
Buffer:
20 mM Hepes pH 7.5; 150 mM NaCl, 0.001 % LMNG
Concentration:
batch dependent
Storage Conditions:
–80°C. Avoid freeze-thaw cycles
Ncbi Official Symbol:
S
Additional Names:
SARS-CoV-2 (COVID-19) Spike Recombinant Protein: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Surface Glycoprotein, Spike protein
Ncbi Gene Id Number:
43740568
Background:
All viruses undergo fast mutations and adapt quickly to the countermeasures that the immune systems creates. SARS-CoV-2 of the COVID-19 pandemic is no exception to this. During the pandemic multiple mutant strains arose. To help the sciencific community to combat these mutants ProSci offers the SPIKE protein of the mutants in full-length and active in their native trimeric form, stabilized with the LMNG detergent.; This is the spike protein of the mutant strain B.1.351, also commonly known as the "South Africa mutant".

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

21-819

List Size:
0.025 mg

List Price:

$631.00

Datasheet
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