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<strong>Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) NSP10 in COVID-19 Patient Lung</strong><br> Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) NSP10 antibody (9179, 0.5 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 NSP10 protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.
<strong>Figure 2 ELISA Validation with SARS-CoV-2 (COVID-19) NSP10 Protein </strong><br>Antibodies: SARS-CoV-2 (COVID-19) NSP10 Antibody, 9179 A direct ELISA was performed using SARS-CoV-2 NSP10 recombinant protein (10-408) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP10 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL.
<strong>Figure 3 Western Blot Validation with SARS-CoV-2 (COVID-19) NSP10 Protein</strong><br>Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) NSP10 recombinant protein (10-408).Antibodies: SARS-CoV-2 (COVID-19) NSP10, 9179, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 1 μg/mL Lane 2: 2 μg/mLLane 3: 4 μg/mL
Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) NSP10 in COVID-19 Patient Lung
Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) NSP10 antibody (9179, 0.5 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 NSP10 protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.
New
Trial Size

SARS-CoV-2 (COVID-19) NSP10 Antibody

CATALOG NUMBER: 9179

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Virus
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Virus
Homology:
Predicted reactivity based on immunogen sequence: SARS-CoV NSP10 protein: (80%)
Immunogen:
Anti-SARS-CoV-2 (COVID-19) NSP10 antibody (9179) was raised against a peptide corresponding to 10 amino acids near the carboxyl terminus of SARS-CoV-2 (COVID-19) NSP10 protein.
The immunogen is located in the last 50 amino acids of SARS-CoV-2 (COVID-19) NSP10.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
WB 1-4 μg/mL; IHC: 0.5 μg/mL.
Antibody validated: Immunohistochemistry in human samples. SARS-CoV-2 (COVID-19) NSP10 antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA. It can detect SARS-CoV-2 NSP10 recombinant protein by ELISA and WB. All other applications and species not yet tested.
Purification:
SARS-CoV-2 (COVID-19) NSP10 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SARS-CoV-2 (COVID-19) NSP10 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SARS-CoV-2 (COVID-19) NSP10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Non-structural protein 10
Additional Names:
NSP10, Growth factor-like peptide, GFL
Protein Accession Number:
YP_009742617.1
Protein Gi Number:
1826688927
Ncbi Gene Id Number:
43740578
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019–20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. NSP10 plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities. Therefore it plays an essential role in viral mRNAs cap methylation (3).
Background Reference 1:
Gorbalenya. bioRxiv: 2020.
Background Reference 2:
Hui et al. Int J Infect Dis. 2020;91:264-266.
Background Reference 3:
Bouvet et. al. Proc. Natl. Acad. Sci. U.S.A. 2012;109:9372-9377

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

9179

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

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