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<strong>Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) NSP14 in COVID-19 Patient Lung</strong><br> Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) NSP14 antibody (9185, 0.5 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 NSP14 protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.
<strong>Figure 2 ELISA Validation </strong><br>Antibodies: SARS-CoV-2 (COVID-19) NSP14 (ExoN) Antibody, 9185. A direct ELISA was performed using immunogen peptide (9185P) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP14 (ExoN) antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000ng/mL.
Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) NSP14 in COVID-19 Patient Lung
Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) NSP14 antibody (9185, 0.5 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 NSP14 protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.
New
Trial Size

SARS-CoV-2 (COVID-19) NSP14 (ExoN) Antibody

CATALOG NUMBER: 9185

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Rabbit
Species Reactivity:
Virus
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Virus
Homology:
Predicted reactivity based on immunogen sequence: SARS-CoV NSP14 (ExoN) protein: (79%).
Immunogen:
Anti-SARS-CoV-2 (COVID-19) NSP14 (ExoN) antibody (9185) was raised against a peptide corresponding to 14 amino acids near the center of SARS-CoV-2 (COVID-19) NSP14 (ExoN) protein.
The immunogen is located between 120-170 amino acids of SARS-CoV-2 (COVID-19) NSP14 (ExoN).
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
IHC: 0.5 μg/mL .
Antibody validated: Immunohistochemistry in human samples. SARS-CoV-2 (COVID-19) NSP14 (ExoN) antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA. All other applications and species not yet tested
Purification:
SARS-CoV-2 (COVID-19) NSP14 (ExoN) Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SARS-CoV-2 (COVID-19) NSP14 (ExoN) Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SARS-CoV-2 (COVID-19) NSP14 (ExoN) antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ExoN
Additional Names:
Proofreading exoribonuclease, Guanine-N7 methyltransferase, Non-structural protein 14, NSP14.
Protein Accession Number:
YP_009725309.1
Protein Gi Number:
1802476817
Ncbi Gene Id Number:
43740578
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019–20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. NSP14 (ExoN) is an enzyme possessing two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity (3,4,5). It acts as a proofreading exoribonuclease for RNA replication, thereby lowering The sensitivity of the virus to RNA mutagens(6,7,8).
Background Reference 1:
Gorbalenya. bioRxiv: 2020.
Background Reference 2:
Hui et al. Int J Infect Dis. 2020;91:264-266.
Background Reference 3:
Minskaia et. al. Proc. Natl. Acad. Sci. U.S.A 2006; 103:5108-5113
Background Reference 4:
Bouvet et. al. PLoS Pathog. 2010; 6:E1000863-E1000863
Background Reference 5:
Bouvet et. al. Proc. Natl. Acad. Sci. U.S.A. 2012;109:9372-9377
Background Reference 6:
Denison et. al. RNA Biol. 2011;8:270-279
Background Reference 7:
Smith et. al. PLoS Pathog. 2013;9:e1003565-e1003565
Background Reference 8:
Agostini et. al. MBio 2018; 9:0-0

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

9185

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

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