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<strong>Figure 1 ELISA Validation with SARS-CoV-2 (COVID-19) NSP15 Protein </strong><br>Antibodies: SARS-CoV-2 (COVID-19) NSP15 Antibody, 9269. A direct ELISA was performed using SARS-CoV-2 NSP15 recombinant protein (10-420) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP15 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL
<strong>Figure 2 Western Blot Validation with SARS-CoV-2 (COVID-19) NSP15 Protein</strong><br>Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) NSP15 recombinant protein (10-420).Antibodies: SARS-CoV-2 (COVID-19) NSP15, 9269, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 0.5 μg/mL and Lane 2: 1 μg/mL
Figure 1 ELISA Validation with SARS-CoV-2 (COVID-19) NSP15 Protein
Antibodies: SARS-CoV-2 (COVID-19) NSP15 Antibody, 9269. A direct ELISA was performed using SARS-CoV-2 NSP15 recombinant protein (10-420) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP15 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL
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SARS-CoV-2 (COVID-19) NSP15 Antibody

CATALOG NUMBER: 9269

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Virus
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Virus
Homology:
Predicted reactivity based on immunogen sequence: SARS-CoV NSP15 protein: (100%).
Immunogen:
Anti-SARS-CoV-2 (COVID-19) NSP15 antibody (9269) was raised against a peptide corresponding to 15 amino acids near the carboxyl terminus of SARS-CoV-2 (COVID-19) NSP15 protein.
The immunogen is located within the last 50 amino acids of the SARS-CoV-2 (COVID-19) NSP15 protein.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB: 0.5-1 μg/mL
Antibody validated: SARS-CoV-2 (COVID-19) NSP15 antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA. It can detect SARS-CoV-2 NSP15 recombinant protein by ELISA and WB. All other applications and species not yet tested.
Purification:
SARS-CoV-2 (COVID-19) NSP15 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SARS-CoV-2 (COVID-19) NSP15 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SARS-CoV-2 (COVID-19) NSP15 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NSP15
Additional Names:
Uridylate-specific endoribonuclease, NendoU, NSP15, Non-structure protein 15
Protein Accession Number:
6VWW_B
Protein Gi Number:
1851775710
Ncbi Gene Id Number:
43740578
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019–20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. NSP15 is a Mn2+-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond.
Background Reference 1:
Gorbalenya. bioRxiv: 2020.
Background Reference 2:
Hui et al. Int J Infect Dis. 2020;91:264-266.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

9269

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

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