Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

<strong>Figure 1 ELISA Validation </strong><br>Antibodies: SARS-CoV-2 (COVID-19) NSP4 Antibody, 9175. A direct ELISA was performed using immunogen peptide (9175P) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP4 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000ng/mL
Figure 1 ELISA Validation
Antibodies: SARS-CoV-2 (COVID-19) NSP4 Antibody, 9175. A direct ELISA was performed using immunogen peptide (9175P) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP4 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000ng/mL
New
Trial Size

SARS-CoV-2 (COVID-19) NSP4 Antibody

CATALOG NUMBER: 9175

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Rabbit
Species Reactivity:
Virus
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Virus
Homology:
Predicted reactivity based on immunogen sequence: SARS-CoV NSP4 protein: (85%)
Immunogen:
Anti-SARS-CoV-2 (COVID-19) NSP4 antibody (9173) was raised against a peptide corresponding to 15 amino acids near the center of SARS-CoV-2 (COVID-19) NSP4 protein.
The immunogen is located within 50-100 amino acids of the SARS-CoV-2 (COVID-19) NSP4 protein.
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Antibody validated: SARS-CoV-2 (COVID-19) NSP4 antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA.
Purification:
SARS-CoV-2 (COVID-19) NSP4 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SARS-CoV-2 (COVID-19) NSP4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SARS-CoV-2 (COVID-19) NSP4 antibody can be stored at 4 ° C for three months and -20 ° C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Non-structural protein 4
Additional Names:
NSP4
Protein Accession Number:
YP_009742609.1
Protein Gi Number:
1826688921
Ncbi Gene Id Number:
43740578
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019–20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. NSP4 participates in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication (3)(4).
Background Reference 1:
Gorbalenya. bioRxiv: 2020.
Background Reference 2:
Hui et al. Int J Infect Dis. 2020;91:264-266.
Background Reference 3:
Angelini et. al. DNA Cell Biol. 33:122-127 (2014)
Background Reference 4:
Angelini et. al. MBio 4:0-0(2013)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

9175

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369