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<strong>Figure 1 ELISA Validation </strong><br>Antibodies: SARS-CoV-2 (COVID-19) NSP6 Antibody, 9177. A direct ELISA was performed using immunogen peptide (9177P) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP6 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000ng/mL.
Figure 1 ELISA Validation
Antibodies: SARS-CoV-2 (COVID-19) NSP6 Antibody, 9177. A direct ELISA was performed using immunogen peptide (9177P) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP6 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000ng/mL.
New
Trial Size

SARS-CoV-2 (COVID-19) NSP6 Antibody

CATALOG NUMBER: 9177

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Rabbit
Species Reactivity:
Virus
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Virus
Homology:
Predicted reactivity based on immunogen sequence: SARS-CoV NSP6 protein: (94%)
Immunogen:
Anti-SARS-CoV-2 (COVID-19) NSP6 antibody (9177) was raised against a peptide corresponding to 18 amino acids in the center of SARS-CoV-2 (COVID-19) NSP6 protein.
The immunogen is located in between 230-280 amino acids of the SARS-CoV-2 (COVID-19) NSP6 protein.
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Antibody validated: SARS-CoV-2 (COVID-19) NSP6 antibody can detect 2ng of free peptide at 1 μg/mL in ELISA.
Purification:
SARS-CoV-2 (COVID-19) NSP6 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SARS-CoV-2 (COVID-19) NSP6 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SARS-CoV-2 (COVID-19) NSP6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Non-structural protein 6
Additional Names:
NSP6
Protein Accession Number:
YP_009742613.1
Protein Gi Number:
1826688923
Ncbi Gene Id Number:
43740578
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019–20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. NSP6 plays a role in the initial induction of autophagosomes from host reticulum endoplasmic. Later, it limits the expansion of these phagosomes that are no longer able to deliver viral components to lysosomes (3,4).
Background Reference 1:
Gorbalenya. bioRxiv: 2020.
Background Reference 2:
Hui et al. Int J Infect Dis. 2020;91:264-266.
Background Reference 3:
Angelini et. al. DNA Cell Biol. 33:122-127 (2014)
Background Reference 4:
Cottam et. al. Autophagy 2014; 10:1426-1441

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

9177

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

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