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<strong>Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) NSP7 in COVID-19 Patient Lung</strong><br> Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) NSP7 antibody (9155, 0.5 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 NSP7 protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.
<strong>Figure 2 ELISA Validation with SARS-CoV-2 (COVID-19) NSP7 Protein </strong><br>Antibodies: SARS-CoV-2 (COVID-19) NSP7 Antibody, 9155. A direct ELISA was performed using SARS-CoV-2 NSP7 recombinant protein as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP7 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 1000 ng/mL
Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) NSP7 in COVID-19 Patient Lung
Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) NSP7 antibody (9155, 0.5 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 NSP7 protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.
New
Trial Size

SARS-CoV-2 (COVID-19) NSP7 Antibody

CATALOG NUMBER: 9155

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Rabbit
Species Reactivity:
Virus
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Virus
Homology:
Predicted reactivity based on immunogen sequence: SARS-CoV NSP7 protein: (100%)
Immunogen:
Anti-SARS-CoV-2 (COVID-19) NSP7 antibody (9155) was raised against a peptide corresponding to 12 amino acids near the carboxyl terminus of the SARS-CoV-2 (COVID-19) NSP7 protein.
The immunogen is located within the last 50 amino acids of the SARS-CoV-2 (COVID-19) NSP7 protein.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
IHC: 0.5 μg/ml.
Antibody validated: Immunohistochemistry in human samples. SARS-CoV-2 (COVID-19) NSP7 antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA. It can detect SARS-CoV-2 NSP7 recombinant protein by ELISA. All other applications and species not yet tested.
Purification:
SARS-CoV-2 (COVID-19) NSP7 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SARS-CoV-2 (COVID-19) NSP7 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SARS-CoV-2 (COVID-19) NSP7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Non-structural protein 7
Additional Names:
NSP7
Protein Accession Number:
YP_009742614.1
Protein Gi Number:
1826688924
Ncbi Gene Id Number:
43740578
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019–20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. NSP7 plays a role in viral RNA synthesis (3,4,5). It forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, it may synthesize substantially longer products than oligonucleotide primers.
Background Reference 1:
Gorbalenya. bioRxiv: 2020.
Background Reference 2:
Hui et al. Int J Infect Dis. 2020;91:264-266.
Background Reference 3:
Gao et. al Science 2020;0:0-0
Background Reference 4:
Yin et. al Science 2020;0:0-0
Background Reference 5:
Hillen et. al. Nature 2020;0:0-0

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

9155

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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