Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

<strong>Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Nucleocapsid in COVID-19 Patient Lung</strong><br> Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Nucleocapsid antibody (10-751, 5 ng/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Nucleocapsid protein was observed in the macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.
Microtiter wells were coated with SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) and SARS-CoV NP at 1 ug/mL. Purified rabbit monoclonal antibody 84C4a wasserially diluted 1:2 starting at 1 ug/mL, and shows very strong and specific binding to COVID-19 NP antigen, with no significant cross-reactivity to SARS-CoV NP antigen.
A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies: 84C4a as a capture antibody and75G5a-biotin as a detection antibody. COVID-19 NP was serially diluted 1:2 starting at 25 ng/mL. Rabbit monoclonal antibodies 84C4a and 75G5a-biotin detectedCOVID-19 NP antigen at very high sensitivity as low as 49 pg/mL (4.9 pg).
Western blot analysis of SARS-CoV2 and SARS-CoV nucleocapsid protein (50 ng) probed with 1 ug/mL CoV2NP rabbit monoclonal antibody (84C4a), CoV2NP-84C4.
Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Nucleocapsid in COVID-19 Patient Lung
Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Nucleocapsid antibody (10-751, 5 ng/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Nucleocapsid protein was observed in the macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.
Recombinant

SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [84C4a]

CATALOG NUMBER: 10-751

Clonality:
Recombinant Monoclonal
Tested Applications:
ELISA
Host Species:
Rabbit
Species Reactivity:
Virus
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Virus
Homology:
Highly-specific to SARS-CoV-2 nucleoprotein; does not cross-react with nucleoprotein from SARS-CoV or other coronaviruses
Immunogen:
SARS-CoV-2 nucleoprotein (NP) specific peptide
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Paired with 75G5a, used for specific, high-sensitivity detection of SARS-CoV-2 nucleocapsid protein (NP).
Purification:
SARS-CoV-2 (COVID-19) Nucleoprotein Antibody [84C4a] is affinity chromatography purified via peptide column.
Clonality:
Recombinant Monoclonal
Isotype:
IgG1k
Physical State:
Liquid
Buffer:
1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Concentration:
batch dependent
Storage Conditions:
SARS-CoV-2 (COVID-19) Nucleoprotein Antibody [84C4a] can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
N
Additional Names:
SARS-CoV-2 (COVID-19) Nucleoprotein Antibody [P002A]: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Protein Accession Number:
QHD43423
Protein Gi Number:
1820552696
Ncbi Gene Id Number:
43740575
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
We have leveraged our next-generation rabbit mAb discovery platform to develop recombinant rabbit monoclonal antibodies with extremely high sensitivity and specificity to SARS-CoV-2. These antibodies, which include clones P001A, P002A, and P003A can be paired in sandwich detection assay and used to detect nucleoprotein (NP) antigen from SARS-CoV-2. These antibodies do not cross-react to the highly related SARS-CoV virus or to any other coronaviruses tested. Sandwich ELISA detection using TMB/acid developer reliably detects NP antigen in the pg/mL range, and sensitivity is expected to be even higher when using more sensitive developer strategies. This panel of antibodies can be used as raw materials for diagnostic kits and can be applied to lateral flow systems for diagnostic detection of COVID-19.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

10-751

List Size:
0.1 mg

List Price:

$515.00

Out of stock

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369