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<strong>Figure 1 Western Blot Validation with Recombinant Protein</strong><br>Loading: 1ug of SARS-CoV-2 (COVID-19) nucleocapsid recombinant protein, 10-007, per lane. Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid Monoclonal Antibody, 10-535, 1:2000. Secondary: Goat anti-human IgG HRP conjugate at 1:5000 dilution.
<strong>Figure 2 ELISA Test</strong><br>Coating original concentration: 2 ug/mL, 100 uL/well sample is SARS-CoV-2 (COVID-19) Nucleocapsid Recombinant Protein, 10-007.<br>Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid Monoclonal Antibody, 10-535.<br>Secondary: Goat anti-human IgG HRP conjugate at 1:10000 dilution.<br>Develop: 15min, 100 uL/well.<br>Stop: Stop buffer 50 uL/well.
Figure 1 Western Blot Validation with Recombinant Protein
Loading: 1ug of SARS-CoV-2 (COVID-19) nucleocapsid recombinant protein, 10-007, per lane. Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid Monoclonal Antibody, 10-535, 1:2000. Secondary: Goat anti-human IgG HRP conjugate at 1:5000 dilution.

SARS-CoV-2 (COVID-19) Nucleocapsid Monoclonal Antibody [N001]

CATALOG NUMBER: 10-535

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Human
Species Reactivity:
Virus
Conjugate:
Unconjugated
Specifications
Host Species:
Human
Species Reactivity:
Virus
Immunogen:
Nucleocapsid recombinant protein
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA: 1:160000; WB: 1:2000~3000
Purification:
Greater than 95% as determined by reducing SDS-PAGE.
Clonality:
Monoclonal
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 10% glycerol, pH7.2
Concentration:
batch dependent
Storage Conditions:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Ncbi Official Symbol:
N
Additional Names:
SARS-CoV-2 (COVID-19, 2019-nCoV) Nucleocapsid Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Nucleocapsid Phosphoprotein, Nucleocapsid protein
Protein Accession Number:
QHD43423.2
Ncbi Gene Id Number:
43740575
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Fully humanized monoclonal antibody paired with human monoclonal antibody LOCUS:QHD43423 419aa linear VRL 18-MAR-2020 DEFINITION:nucleocapsid phosphoprotein [Severe acute respiratory syndrome coronavirus 2]. ACCESSION:QHD43423 VERSION:QHD43423.2 DBSOURCE:accession MN908947.3 SOURCE:Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ORGANISM:Viruses; Riboviria; Nidovirales; Cornidovirineae; Coronaviridae;Orthocoronavirinae; Betacoronavirus; Sarbecovirus.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

10-535

List Size:
0.1 mg

List Price:

$556.00

Datasheet
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