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SARS-CoV-2 Nucleocapsid protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized SARS-CoV-2 Nucleocapsid protein, His Tag at 2 ug/mL (100 uL/well) can bind 2019-nCoV NP Antibody, Rabbit MAb (CLN27) with a linear range of 0.02-0.6 ng/mL (QC tested).
SARS-CoV-2 Nucleocapsid protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SARS-CoV-2 (COVID-19) Nucleocapsid Recombinant Protein

CATALOG NUMBER: 11-214

Tested Applications:
ELISA, WB
Specifications
source-species:
E. coli
Species:
SARS-CoV-2
source-species:
E. coli
Recombinant Protein Sequence:
Met 1 - Ala 419
Fusion Tag:
His Tag
Tested Applications:
ELISA, WB
Application Note:
This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 51.0 kDa. The protein migrates as 53-55 kDa under reducing (R) condition (SDS-PAGE).
Predicted Molecular Weight:
51.0 kDa
Purity:
>90% as determined by SDS-PAGE.
physical-state:
Liquid
Buffer:
10 mM PB, Arginine, pH7.4
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
N
Additional Names:
Nucleocapsid protein,NP,Protein N,COVID-19
Protein Accession Number:
QHO62115.1
Ncbi Gene Id Number:
43740575
Background:
Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.
Background Reference 1:
Reuter M, et al. Virus Genes. 2018. 54:5-16.
Background Reference 2:
Grunewald ME, et al. Virology. 2018. 517:62-68.
Background Reference 3:
Jeeva S, et al. PLoS One. 2017. 12(9):e0184935.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

11-214

List Size:
0.2 mg

List Price:

$979.00

Datasheet
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