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<strong>Figure 1 ELISA Validation </strong><br>Antibodies: SARS-CoV-2 (COVID-19) ORF10 Antibody, 9293. A direct ELISA was performed using SARS-CoV-2 ORF10 immunogen peptide (9293P)) as coating antigen and the anti-SARS-CoV-2 (COVID-19) ORF10 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL
Figure 1 ELISA Validation
Antibodies: SARS-CoV-2 (COVID-19) ORF10 Antibody, 9293. A direct ELISA was performed using SARS-CoV-2 ORF10 immunogen peptide (9293P)) as coating antigen and the anti-SARS-CoV-2 (COVID-19) ORF10 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL
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SARS-CoV-2 (COVID-19) ORF10 Antibody

CATALOG NUMBER: 9293

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Rabbit
Species Reactivity:
Virus
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Virus
Immunogen:
Anti-SARS-CoV-2 (COVID-19) ORF10 antibody (9293) was raised against a peptide corresponding to 13 amino acids near the carboxyl terminus of SARS-CoV-2 (COVID-19) ORF10 protein.
The immunogen is located within the last 50 amino acids of the SARS-CoV-2 (COVID-19) ORF10 protein.
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Antibody validated: SARS-CoV-2 (COVID-19) ORF10 antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA. All other applications and species not yet tested.
Specificity:
ORF10 Antibody is predicted to not cross-react with other coronavirus family members.
Predicted Molecular Weight:
4kD
Purification:
SARS-CoV-2 (COVID-19) ORF10 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SARS-CoV-2 (COVID-19) ORF10 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SARS-CoV-2 (COVID-19) ORF10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ORF10
Additional Names:
ORF10 protein, ORF10
Protein Accession Number:
QSJ03260.1
Protein Gi Number:
1996412886
Ncbi Gene Id Number:
43740576
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019–20 coronavirus outbreak (2). SARS-CoV-2 virus proteins include structural proteins, non-structural proteins and accessory factors. The structure of SARS-CoV-2 consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. SARS-CoV-2 non-structural protein is ORF1ab that consists of 16 proteins (nsp1-nsp16), while accessory factors include ORF3a, ORF3b, ORF6, ORF7a, ORF7b, ORF8, ORF9b, ORF10 and ORF10.
Background Reference 1:
Gorbalenya. bioRxiv: 2020.
Background Reference 2:
Hui et al. Int J Infect Dis. 2020;91:264-266.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

9293

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

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