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<strong>Figure 1 Immunohistochemistry Validation of Omicron Variant Spike Specific Antibody on a Nasal Swab Sample from a Omicron COVID-19 Patient.</strong><br> Immunohistochemical analysis of 4% paraformaldehyde-fixed cells from a nasal swab from a patient infected with the Omicron variant stained with Omicron specific antibody (9793) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 2 Immunofluorescence Validation of Omicron Variant Spike Specific Antibody on a Nasal Swab Sample from a Patient Infected with the Omicron Variant. </strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed patient nasal swab sample labeling spike with 9793 and nucleocapsid with 35-722 at 10 μg/mL for each, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution for spike (9793,green) and goat anti-mouse IgG secondary antibody at 1/500 dilution for nucleocapsid (35-722, red) with DAPI counter staining (blue).
<strong>Figure 3 SARS-CoV-2 Omicron Variant Spike Antibodies Specifically Detect Omicron Variant Spike S1 Protein in an ELISA </strong><br>Coating Antigen: SARS-CoV-2 spike S1 proteins of WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529), 1 μg/mL, incubated at 4 °C overnight.Detection Antibodies: SARS-CoV-2 Omicron Variant Spike antibody, 9793, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr.
<strong>Figure 4 WB Validation of of Omicron Variant Spike Antibodies with SARS-CoV-2 Omicron Variant Spike S1 Protein </strong><br>Loading: 30 ng of SARS-CoV-2 spike S1 proteins, including WT, Delta variant (B.1.617.2) and Omicron variant (B.1.1.529).Detection Antibodies: SARS-CoV-2 Omicron Variant Spike antibody, 9793, 2 μg/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr.<strong>SARS-CoV-2 Omicron Variant Spike antibody (9793) specifically detects Omicron variant spike S1 protein (95-129), but not spike S1 protein of WT (10-300) and Delta (95-127). </strong>
<strong>Figure 5 ELISA Validation of Omicron Variant Spike S1 Antibodies with SARS-CoV-2 Omicron Variant Spike S1 Protein </strong><br>Coating Antigen: SARS-CoV-2 spike S1 proteins, WT and Omicron variant, 1 μg/mL, incubated at 4 °C overnight.Detection Antibodies: SARS-CoV-2 Omicron spike antibody, 9793, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr.<strong>SARS-CoV-2 Omicron spike antibody specifically detects spike S1 protein of Omicron variant (95-129), but not WT (10-300). </strong>
<strong>Figure 6 WB Validation of of Omicron Variant Spike Antibodies with SARS-CoV-2 Variant Spike S1 Proteins </strong><br>Loading: 30 ng of SARS-CoV-2 spike S1 proteins, including WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529),Detection Antibodies: SARS-CoV-2 Omicron Variant Spike antibody, 9793, 2 μg/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr.<strong>SARS-CoV-2 Omicron Variant Spike antibody (9793) specifically detects Omicron variant spike S1 protein (95-129), but not spike S1 protein of WT (10-300) and all other variants. </strong>
Figure 1 Immunohistochemistry Validation of Omicron Variant Spike Specific Antibody on a Nasal Swab Sample from a Omicron COVID-19 Patient.
Immunohistochemical analysis of 4% paraformaldehyde-fixed cells from a nasal swab from a patient infected with the Omicron variant stained with Omicron specific antibody (9793) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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SARS-CoV-2 (COVID-19) Spike G142D Δ143-145VYY Antibody (Omicron)

CATALOG NUMBER: 9793

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Virus
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Virus
Homology:
Predicted reactivity based on immunogen sequence: SARS-CoV Spike proteins: (33.3%)
Immunogen:
Anti-SARS-CoV-2 Omicron variant specific Spike (G142D Δ143-145VYY) antibody(9793) was raised against a peptide corresponding to 12 amino acids near the amino terminal domain of SARS-CoV-2 Omicron variant (B.1.1.529) Spike protein.

The immunogen is located within 130-180 amino acids of SARS-CoV-2 Omicron variant (B.1.1.529) Spike protein.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
WB: 2 μg/mL; ICC: 1 μg/mL; IF: 10 μg/mL.

Antibody validated: Western Blot, Immunocytochemistry and Immunofluorescence staining in human samples. Anti-SARS-CoV-2 Omicron variant Spike antibody specifically detects Spike S1 protein of Omicron Variant (B.1.1.529), but not WT and other variant by ELISA and WB. Other applications and species not yet tested.
Specificity:
It can only detect SARS-CoV-2 Omicron Variant (B.1.1.529) Spike protein and does not cross-react with the spike proteins of other variants.
Purification:
Anti-SARS-CoV-2 Omicron Variant Spike antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Anti-SARS-CoV-2 Omicron Variant Spike antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SARS-CoV-2 Omicron Variant Spike antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
S
Additional Names:
SARS-CoV-2 Omicron Variant Spike antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Surface Glycoprotein, Spike protein, Omicron Variant
Protein Accession Number:
QHD43416
Protein Gi Number:
1791269090
Ncbi Gene Id Number:
43740568
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SARS-CoV-2 Omicron variant, a variant of concern (VOC), known as B.1.1.529, was detected in South Africa at the end of November in 2021. However, it rapidly spread all over of the world and now it is the dominant variant in the world, which account for more than 90% of the new cases. Omicron variant spike protein carries around 30 amino acid changes, including mutations, deletions and insertions, 15 of which are in the receptor binding domain (RBD). Enhanced transmission of the Omicron variant was observed globally, which is at least 70 times more contagious than the other variants. The Omicron variant affects the effectiveness of COVID-19 vaccine and is resistant to neutralization (monoclonal antibody treatments) to a large extent.
Background Reference 1:
Gowrisankar et al. European Physical Journal Plus, 2022, 137(1), 100.
Background Reference 2:
Zhang et al. PNAS of U.S.A. 2021, 118(47):e2109905118.
Background Reference 3:
Cameroni et al. Nature 2021.
Background Reference 4:
Rossler et al. NEJM 2022.
Background Reference 5:
Muik et al. Science 2022.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

9793

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

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