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<strong>Figure 1 Immunohistochemistry Validation of Spike in Delta Variant COVID-19 Patient Lung Tissue</strong><br> Immunohistochemical analysis of paraffin-embedded patient lung tissue using anti-Spike P681R antibody (PM-9680) at 20 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. <strong>Strong spike protein signal was observed in the Delta variant COVID-19 patient lung, but not in WT COVID-19 patient lung. </strong>
<strong>Figure 2 SARS-Cov-2 Spike P681R (Delta Variant) Antibodies Specifically Detect Delta Variant Spike S1 Protein in an ELISA </strong><br>Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 μg/mL, incubated at 4 °C overnight.Detection Antibodies: SARS-CoV-2 Spike P681R (Delta Variant) antibody, PM-9680, 1 μg/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.
<strong>Figure 3 ELISA Validation of Delta Variant Spike S1 Antibodies with SARS-CoV-2 Delta Variant Spike S1 Protein </strong><br>Coating Antigen: SARS-CoV-2 spike S1 proteins, WT, Alpha and Delta variants, 1 μg/mL, incubated at 4 °C overnight.Detection Antibodies: SARS-CoV-2 Spike P681R (Delta Variant) antibody, PM-9680, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.<strong>SARS-CoV-2 Spike P681R (Delta Variant) antibody specifically detects spike S1 protein of Delta variant, but not WT and Alpha variant. </strong>
<strong>Figure 4 ELISA Validation of Delta Variant Spike S1 Antibodies with Mutant and WT Peptide </strong><br>Coating Antigen: SARS-CoV-2 spike S1 peptides: WT (9091P), Alpha variant (9359P), and Delta variant (9673P), 1 μg/mL, incubated at 4 °C overnight.Detection Antibodies: SARS-CoV-2 delta spike P681R antibody, PM-9680, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.<strong>SARS-CoV-2 Spike P681R (Delta Variant) antibody detects Delta variant spike S1 peptide (681R, 9673P), but not WT peptide (681P, 9091P) and Alpha variant spike S1 peptide (681H, 9359P). </strong>
Figure 1 Immunohistochemistry Validation of Spike in Delta Variant COVID-19 Patient Lung Tissue
Immunohistochemical analysis of paraffin-embedded patient lung tissue using anti-Spike P681R antibody (PM-9680) at 20 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal was observed in the Delta variant COVID-19 patient lung, but not in WT COVID-19 patient lung.
New
Trial Size

SARS-CoV-2 (COVID-19) Spike P681R Antibody [7E3C5] (Delta Variant)

CATALOG NUMBER: PM-9680

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC-P
Host Species:
Mouse
Species Reactivity:
Virus
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Virus
Homology:
Predicted reactivity based on immunogen sequence: SARS-CoV Spike proteins: (25%)
Immunogen:
Anti-SARS-CoV-2 Spike 681R (Delta Variant) antibody (PM-9680) was raised against a peptide corresponding to 12 amino acids near the cleavage site of SARS-CoV-2 Delta variant Spike protein.

The immunogen is located within 650-700 amino acids of SARS-CoV-2 Delta variant Spike protein.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P
Application Note:
IHC: 20 μg/mL.

Antibody validated: IHC in human samples. Anti-SARS-CoV-2 Spike P681R (Delta Variant) antibody specifically detects SARS-CoV-2 Delta Variant Spike S1 protein, but not SARS-CoV-2 WT and other variant Spike S1 protein by ELISA. All other applications and species not yet tested.
Specificity:
It can only detect SARS-CoV-2 Delta Variant Spike S1 protein and does not cross-react with the spike protein of other variants.
Purification:
Anti-SARS-CoV-2 Spike P681R (Delta Variant) antibody is Protein G purified.
Clonality:
Monoclonal
Isotype:
IgG2
physical-state:
Liquid
Buffer:
SARS-CoV-2 Spike P681R (Delta Variant) antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SARS-CoV-2 Spike P681R (Delta Variant) antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
S
Additional Names:
Anti-SARS-CoV-2 Spike P681R (Delta Variant) antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Surface Glycoprotein, Spike protein, Delta variant
Protein Accession Number:
QHD43416
Protein Gi Number:
1791269090
Ncbi Gene Id Number:
43740568
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SARS-CoV-2 delta variant, a variant of concern (VOC), known as B.1.617.2, was detected in India in October of 2020. However, it rapidly spread all over of the world and now it is the dominant variant in the world, which account for more than 99% of the cases. This variant carries at least 13 mutations in spike protein across the sub lineages, including L452R, D614G, P681R and K417N, which can increase the affinity to the human ACE2 receptor. Enhanced transmission of the Delta variant was observed globally, which is at least 2.5 times more contagious as the other variants. The Delta variant affects the effectiveness of COVID19 vaccine and is resistant to neutralization to some extent.
Background Reference 1:
Zhang et al. JAMA 2021, 325 (13): 1324-26.
Background Reference 2:
Reardon. Nature 2021.
Background Reference 3:
Delphine. Nature 2021, 596 (7871): 276-280.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

PM-9680

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

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