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Antibody used at 1 μg/ml in WB.
Antibody used at 1 μg/ml in WB.

SARS-CoV-2 (COVID-19) Spike RBD domain Antibody

CATALOG NUMBER: 20-259

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Sheep
Species Reactivity:
Virus
Conjugate:
Unconjugated
Specifications
Host Species:
Sheep
Species Reactivity:
Virus
Immunogen:
MBP-SARS-CoV2 S Protein RBD
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Detection Antibodies: 1 μg/ml Ab, 5% BSA in TBS-T, 4°C overnight
Blocking: 5% milk in TBS-T, 1h room temp
Secondary Antibodies: 1:15,000 donkey anti-sheep, 1:15,000 donkey anti-mouse, in TBS-T, 1h room temp
Wash: TBS-T, 4x 10 min
Purification:
Antibodies are affinity purified against the antigen and eluted with low pH.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Antibody supplied in PBS.
Concentration:
batch dependent
Storage Conditions:
Aliquot and store at -20°C. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
S
Additional Names:
SARS-CoV-2-S Protein RBD domain, SARS-CoV2-S Protein RBD domain, SARS-CoV2-Spike Protein RBD domain, SARS-CoV-2-Spike Protein RBD domain
Ncbi Gene Id Number:
43740568
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Coronavirus envelope (E) proteins are short (1 residues) polypeptides that contain at least one transmembrane (TM) domain and a cluster of 2-3 juxtamembrane cysteines. These proteins are involved in viral morphogenesis and tropism, and their absence leads in some cases to aberrant virions, or to viral attenuation. In common to other viroporins, coronavirus envelope proteins increase membrane permeability to ions, plays a central role in virus morphogenesis and assembly. Acts as a viroporin and self-assembles in host membranes forming pentameric protein-lipid pores that allow ion transport. Also plays a role in the induction of apoptosis. Activates the host NLRP3 inflammasome, leading to IL-1beta overproduction.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

20-259

List Size:
0.1 mg

List Price:

$565.00

Out of stock

Datasheet
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