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<strong>Figure 1 Western Blot Validation with Recombinant Protein</strong><br>Loading: 10ug of recombinant protein per lane. Lane 1: 10-008 and Lane 2: 10-011. Antibodies: SARS-CoV-2 (COVID-19) Spike-ECD/RBD Monoclonal, 10-552, 1:1000. Secondary: Goat anti-human IgG HRP conjugate at 1:5000 dilution.
<strong>Figure 2 ELISA Test</strong><br>Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 (COVID-19) Spike-ECD Recombinant Protein, 10-011, and column 2: SARS-CoV-2 (COVID-19) Spike-RBD Recombinant Protein, 10-008.<br>Antibodies: SARS-CoV-2 (COVID-19) Spike-ECD/RBD Monoclonal Antibody, 10-552.<br>Secondary: Goat anti-human IgG HRP conjugate at 1:10000 dilution.<br>Develop: 15min, 100 uL/well.<br>Stop: Stop buffer 50 uL/well.
Figure 1 Western Blot Validation with Recombinant Protein
Loading: 10ug of recombinant protein per lane. Lane 1: 10-008 and Lane 2: 10-011. Antibodies: SARS-CoV-2 (COVID-19) Spike-ECD/RBD Monoclonal, 10-552, 1:1000. Secondary: Goat anti-human IgG HRP conjugate at 1:5000 dilution.

SARS-CoV-2 (COVID-19) Spike RBD Monoclonal Antibody [B001] (azide free)

CATALOG NUMBER: 10-552

Clonality:
Monoclonal
Tested Applications:
ELISA, Neutralization Assay, WB
Host Species:
Human
Species Reactivity:
Virus
Conjugate:
Unconjugated
Specifications
Host Species:
Human
Species Reactivity:
Virus
Immunogen:
S-RBD recombinant protein
Conjugate:
Unconjugated
Tested Applications:
ELISA, Neutralization Assay, WB
Application Note:
ELISA: 1:320000; WB: 1:1000~2000
Purification:
Greater than 95% as determined by reducing SDS-PAGE.
Clonality:
Monoclonal
physical-state:
Liquid
Buffer:
PBS, pH7.2
Concentration:
batch dependent
Storage Conditions:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Ncbi Official Symbol:
S
Additional Names:
SARS-CoV-2 (COVID-19, 2019-nCoV) Spike Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Surface Glycoprotein, Spike protein
Protein Accession Number:
QHD43416.1
Ncbi Gene Id Number:
43740568
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. SARS CoV-2 Spike Protein derived from 2019-nCoV is composed of S1 domain and S2 domain. The S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

10-552

List Size:
0.1 mg

List Price:

$577.00

Datasheet
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