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<strong> Figure 1 Detection of SARS-CoV-2 Variant Proteins with Spike S1 Antibodies by Direct ELISA</strong> Coating Antigen: SARS-CoV-2 full length spike proteins, including WT, UK variant (B.1.1.7), SA variant (B.1.135) and Brazil (P.1). Dilution: 1-3000 ng/mL. Incubate at 4 ° C overnight.Detection Antibodies: SARS-CoV-2 Spike S1 Antibody, 10-351, 0.1 μg/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.<strong>Spike S1 antibody (10-351) cannot detect SA variant (B.1.135) and Brazil (P.1), both of which carry more mutations in RBD as compared to UK variant (B.1.1.7). </strong>
<strong>Figure 2 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Spike in COVID-19 Patient Lung</strong><br> Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Spike antibody (10-351, 3 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Spike protein was observed in the macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.
<strong>Figure 3 Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Spike S1 Antibodies</strong><br>Antibodies: SARS-CoV-2 (COVID-19) Spike Antibodies, 10-351 and 10-706. A sandwich ELISA was performed using SARS-CoV-2 Spike S1 antibody (10-351, 2ug/ml) as capture antibody, the Spike S1 recombinant protein as the binding protein (97-087), and the anti-SARS-CoV-2 Spike S1 antibody (10-706, 1ug/ml) as the detection antibody. Secondary: Mouse anti-human IgG HRP conjugate (PM6727) at 1:10000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 1.58 ng
<strong>Figure 4 Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Spike S1 Antibodies</strong><br>Antibodies: SARS-CoV-2 (COVID-19) Spike Antibodies, 10-351 and 9083-biotin. A sandwich ELISA was performed using SARS-CoV-2 Spike S1 antibody (10-351, 2ug/ml) as capture antibody, the Spike S1 recombinant protein as the binding protein (97-087), and the anti-SARS-CoV-2 Spike S1 antibody (9083-biotin, 1ug/ml) as the detection antibody. Secondary: Streptavidin-HRP at 1:10000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 1.96 ng
<strong>Figure 5 Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Spike S1 Antibodies</strong><br>Antibodies: SARS-CoV-2 (COVID-19) Spike Antibodies, 10-351 and 9091-biotin. A sandwich ELISA was performed using SARS-CoV-2 Spike S1 antibody (10-351, 2ug/ml) as capture antibody, the Spike S1 recombinant protein as the binding protein (97-087), and the anti-SARS-CoV-2 Spike S1 antibody (9091-biotin, 1ug/ml) as the detection antibody. Secondary: Streptavidin-HRP at 1:10000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 3.14 ng
Figure 1 Detection of SARS-CoV-2 Variant Proteins with Spike S1 Antibodies by Direct ELISA Coating Antigen: SARS-CoV-2 full length spike proteins, including WT, UK variant (B.1.1.7), SA variant (B.1.135) and Brazil (P.1). Dilution: 1-3000 ng/mL. Incubate at 4 ° C overnight.Detection Antibodies: SARS-CoV-2 Spike S1 Antibody, 10-351, 0.1 μg/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.Spike S1 antibody (10-351) cannot detect SA variant (B.1.135) and Brazil (P.1), both of which carry more mutations in RBD as compared to UK variant (B.1.1.7).

SARS-CoV-2 (COVID-19) Spike S1 Antibody [SP185]

CATALOG NUMBER: 10-351

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC
Host Species:
Rabbit
Species Reactivity:
Virus
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Virus
Immunogen:
Recombinant SARS-CoV-2 spike protein, S1 subunit.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Purification:
The antibody was expressed in HEK293F cells, and subsequently obtained from cell supernatant by protein A affinity chromatography.
Clonality:
Monoclonal
Isotype:
IgG, kappa
physical-state:
Liquid
Buffer:
PBS pH7.2
Concentration:
batch dependent
Storage Conditions:
SARS-CoV-2 (COVID-19) Spike S1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
S
Additional Names:
SARS-CoV-2 (COVID-19) Spike S1 Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Surface Glycoprotein, Spike protein
Protein Accession Number:
QHD43416
Protein Gi Number:
1791269090
Ncbi Gene Id Number:
43740568
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

10-351

List Size:
0.1 mg

List Price:

$498.00

Datasheet
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