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Figure 1: Data from our lab regarding SPIKE stabilized and purified in synthetic nanodiscs (Dibma Glycerol).<br>A: SDS of SPIKE in synthetic nanodisc.<br>B: Western blot of Rhot1d4 tagged SPIKE, originated from the SDS Page seen in "A"<br>C: Native protein Gel of purified and stabilized SPIKE in synthetic nanodisc.
Figure 1: Data from our lab regarding SPIKE stabilized and purified in synthetic nanodiscs (Dibma Glycerol).
A: SDS of SPIKE in synthetic nanodisc.
B: Western blot of Rhot1d4 tagged SPIKE, originated from the SDS Page seen in "A"
C: Native protein Gel of purified and stabilized SPIKE in synthetic nanodisc.

SARS CoV-2 full length spike protein in DIBMA Glycerol

CATALOG NUMBER: 21-816

Tested Applications:
ELISA
Specifications
source-species:
HEK293 cells
Species:
SARS-CoV-2 (COVID-19, 2019-nCoV) Spike Protein
source-species:
HEK293 cells
Recombinant Protein Sequence:
Full-length sequence (aa 1 – 1273),
furin cleavage site “RRAR” mutated to “GSAG”;
C-terminal Rho1D4 tag fused with spacer “GSSG” to protein sequence
Fusion Tag:
C-terminal Rho1D4
Tested Applications:
ELISA
Application Note:
This recombinant protein can be used for ELISA assays, Ligand binding assays (e.g. SPR), Biochemical and biophysical analyses. For research use only.
Predicted Molecular Weight:
1286 amino acids (including Rho1D4 tag and linker); 142114 Da
Biological Activity:
not determined
Purity:
>98% as determined by SDS-PAGE, see Fig. 1 A and B. PureCube Rho1D4 Agarose.
physical-state:
Liquid
Buffer:
20 mM Hepes pH 7.5; 150 mM NaCl,: buffer does not contain free DIBMA Glycerol (DIBMA: Diisobutylene-maleic acid)
Concentration:
batch dependent
Storage Conditions:
–80°C. Avoid freeze-thaw cycles
Ncbi Official Symbol:
S
Additional Names:
SARS-CoV-2 (COVID-19) Spike Recombinant Protein: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Surface Glycoprotein, Spike protein
Ncbi Gene Id Number:
43740568
Background:
The coronavirus, also known as SARS-CoV-2, enters the cell by using its surface SPIKE. SPIKE is processed on the cell's surface by TMPRSS2, a serine protease. It then subsequently binds to ACE2 a cell surface receptor. The Native SPIKE protein is a trimer that is located in the coronavirus membrane. Therefore to get pure & native SPIKE the trimer needs to be kept intact. Our lab staff achieved this in three different ways: MSP nanodiscs, based on MSP proteins Detergent Mycelles, as you can see here Synthetic nanodiscs

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

21-816

List Size:
0.025 mg

List Price:

$1,250.00

Datasheet
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