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Western blot analysis of from wild type (WT) and 293T cells transfected with SARS-CoV-2 N Protein using SARS-CoV-2 N Protein Mouse mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 20μg/10μg/5μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 5s.
Immunofluorescence analysis of 293T transfected with SARS-CoV-2 N Protein using SARS-CoV-2 Nucleoprotein mAb mAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Mouse IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immobilized Recombinant 2019-nCoV Nucleocapsid Protein at 1μg/mL (100μL/well) can bind SARS-CoV-2 Nucleoprotein Rabbit mAb with a linear range of 3.12-200ng/mL.
Western blot analysis of from wild type (WT) and 293T cells transfected with SARS-CoV-2 N Protein using SARS-CoV-2 N Protein Mouse mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 20μg/10μg/5μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 5s.

SARS-CoV-2 N Protein Mouse Recombinant Antibody (81-679)

CATALOG NUMBER: 81-679

Clonality:
Monoclonal
Tested Applications:
ELISA, IF/ICC, WB
Host Species:
Mouse
Species Reactivity:
SARS-CoV-2
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
SARS-CoV-2
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF/ICC, WB
Application Note:
WB,1:1000 - 1:6000, IF/ICC,1:50 - 1:200, ELISA,1:30000 - 1:120000
Predicted Molecular Weight:
46kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG1,Kappa
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
Nucleoprotein
Additional Names:
Nucleoprotein; NP; SARS-CoV-2 N Protein
Protein Accession Number:
P59595
Ncbi Gene Id Number:
43740575
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host SMAD3.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

81-679

List Size:
100 uL

List Price:

$477.00

Datasheet
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