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Recombinant SARS-CoV-2 Nucleocapsid Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
The purity of SARS-COV-2 Nucleocapsid Protein with His tag was greater than 95% as determined by SEC-HPLC.
Immobilized Recombinant SARS-COV-2 Nucleocapsid at 2μg/mL (100 μL/well) can bind Anti-Nucleocapsid antibody with a linear range of 0.12-1.64 ng/mL.
Immobilized Recombinant SARS-CoV-2 envelope Protein at 2μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 Nucleocapsid protein, the EC<sub>50</sub> of Recombinant SARS-CoV-2 Nucleocapsid protein is 41.10 ng/mL.
Recombinant SARS-CoV-2 Nucleocapsid Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

SARS-CoV-2 Nucleocapsid Recombinant Protein (85-347)

CATALOG NUMBER: 85-347

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
E. coli
Species:
SARS-CoV-2
source-species:
E. coli
Recombinant Protein Sequence:
Met1-Ala419
Fusion Tag:
N-His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
46.48 kDa
Biological Activity:
1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 Nucleocapsid at 2 μg/mL (100 μL/well) can bind Anti-Nucleocapsid antibody with a linear range of 0.12-1.64 ng/mL.
2.Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 envelope Protein at 2 μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 Nucleocapsid protein, the EC50 of Recombinant SARS-CoV-2 Nucleocapsid protein is 41.10 ng/mL.
Purity:
≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Supplied as a 0.22 μm filtered solution in 20mM Tris, 500mM NaCl, 0.1mM EDTA, 10% glycerol, pH8.0.Contact us for customized product form or formulation.
Storage Conditions:
Store at -70°C. This product is stable at ≤ -70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.
Additional Names:
Nucleoprotein
Protein Accession Number:
QHD43423.2
Ncbi Gene Id Number:
43740575

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-347

List Size:
0.1 mg

List Price:

$420.00

Datasheet
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