Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

<strong>Figure 1 Neutralization Assay of Omicron Variant Pseudovirus by SARS-CoV-2 Omicron Variant Spike Antibody </strong><br>Neutralization antibodies: Anti-SARS-CoV-2 Spike RBD antibodies, dilution: 0.3 - 30,000 ng/mL.<br>Pseudovirus: SARS-CoV-2 Omicron Spike Pseudovirus, 95-201.<br>Infected Cells: ACE2-overexpressing 293T cells<br>Diluted antibody was incubated with Omicron pseudovirus at 37 °C for 1 hr. ACE2-overexpressing 293T cells were added in each well and incubated at 37 °C for 48 hrs. Luminescence reporter reagent was added in each well and the final results were read with the luminescence plate reader. Percent inhibition is calculated based on the RLU value.
<strong>Figure 2 Neutralization Assay of Omicron Variant Pseudovirus by SARS-CoV-2 Omicron Variant Spike Antibody </strong><br>Neutralization antibodies: Anti-SARS-CoV-2 Spike RBD antibodies, 9805, dilution: 0.3 - 30,000 ng/mL.<br>Pseudovirus: SARS-CoV-2 Omicron Spike Pseudovirus, 95-201.<br>Infected Cells: ACE2-overexpressing 293T cells<br>Diluted antibody was incubated with Omicron pseudovirus at 37 °C for 1 hr. ACE2-overexpressing 293T cells were added in each well and incubated at 37 °C for 48 hrs. Luminescence reporter reagent was added in each well and the final results were read with the luminescence plate reader. Percent inhibition is calculated based on the RLU value.
Figure 1 Neutralization Assay of Omicron Variant Pseudovirus by SARS-CoV-2 Omicron Variant Spike Antibody
Neutralization antibodies: Anti-SARS-CoV-2 Spike RBD antibodies, dilution: 0.3 - 30,000 ng/mL.
Pseudovirus: SARS-CoV-2 Omicron Spike Pseudovirus, 95-201.
Infected Cells: ACE2-overexpressing 293T cells
Diluted antibody was incubated with Omicron pseudovirus at 37 °C for 1 hr. ACE2-overexpressing 293T cells were added in each well and incubated at 37 °C for 48 hrs. Luminescence reporter reagent was added in each well and the final results were read with the luminescence plate reader. Percent inhibition is calculated based on the RLU value.
New

SARS-CoV-2 Omicron BA.1 Variant Pseudovirus

CATALOG NUMBER: 95-201

Tested Applications:
Neutralization Assay, Viral Infection
Specifications
source-species:
HEK293 cells
Species:
SARS-CoV-2 (COVID-19) Spike pseudovirus
Original Species Name:
Firefly luciferase
source-species:
HEK293 cells
Recombinant Protein Sequence:
Omicron Variant Spike BA.1 Met 1 - Cys 1255 (A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F)
Tested Applications:
Neutralization Assay, Viral Infection
Application Note:

Protocol: click here to download

1. Require BSL-2 or higher working environment
2. 5 μL per application (96-well plate) with at least 0.3 X 106 RLU / well.
3. Avoid freezing and thawing cycle to ensure the titer of the pseudovirus.
4. This product is for Research Use only
5. Neutralization assay protocol is available
Reporter:
Firefly luciferase
physical-state:
Liquid
Buffer:
DMEM/10% FBS
Size Description:
1.0 mL (0.5mL X 2 vials) for two 96-well plates
Storage Conditions:
The pseudovirus is stable in a liquid state at -70° for 12 months. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
S
Additional Names:
Spike glycoprotein Receptor-binding domain, S glycoprotein, Spike protein, Omicron variant spike, Omicron variant pseudovirus, Omicron, pseudovirus reporter, reporter virus particle
Protein Accession Number:
QHD43416
Protein Gi Number:
1791269090
Ncbi Gene Id Number:
43740568
Background:
SARS-CoV-2 Omicron Pseudovirus uses HIV pseudotyped with SARS-CoV-2 spike protein and firefly luciferase reporter. This pseudovirus effectively infects human ACE2 overexpressing cells and can be used to screen for inhibitors of Spike-ACE2 interaction, determine neutralizing antibody titers, and is a valuable tool for COVID-19 vaccine development as well.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

95-201

List Size:
1.0 mL (0.5mL X 2 vials)

List Price:

$419.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369