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Recombinant SARS-CoV-2 Spike RBD Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
The purity of SARS-COV-2 Spike RBD Protein with His tag (Cat. 85-351) was greater than 95% as determined by SEC-HPLC.
Immobilized SARS-CoV-2 Spike RBD at 2μg/mL (100 μL/well) can bind recombinant Human ACE2 with a linear range of 0.15-3.63 ng/mL.
Recombinant SARS-CoV-2 Spike RBD Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

SARS-CoV-2 Spike RBD Recombinant Protein (85-351)

CATALOG NUMBER: 85-351

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
SARS-CoV-2
source-species:
HEK293 cells
Recombinant Protein Sequence:
Arg319-Phe541
Fusion Tag:
C-His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
25.94 kDa
Biological Activity:
Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 Spike RBD at 2 μg/mL (100 μL/well) can bind recombinant Human ACE2 with a linear range of 0.15-3.63 ng/mL.
Purity:
≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4 or Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.Contact us for customized product form or formulation.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
Envelope,SARS-CoV-2 Spike RBD (N501Y),Spike,Spike ECD,Spike RBD,Spike S1,Spike S2,Spike S2 ECD,S1-RBD protein,NCP-CoV RBD Protein,novel coronavirus RBD Protein,2019-nCoV RBD Protein,S glycoprotein Subunit1 RBD Protein
Protein Accession Number:
YP_009724390.1
Ncbi Gene Id Number:
43740568

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-351

List Size:
0.1 mg

List Price:

$560.00

Datasheet
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