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<strong>Figure 1 ELISA Test </strong><br>Antibodies: SARS-CoV Matrix Antibody, 3527 (1 μg/mL). A direct ELISA was performed using immunogen as coating antigen and the anti-SARS-CoV Matrix antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 2 ng/mL to 1000 ng/mL.
Figure 1 ELISA Test
Antibodies: SARS-CoV Matrix Antibody, 3527 (1 μg/mL). A direct ELISA was performed using immunogen as coating antigen and the anti-SARS-CoV Matrix antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 2 ng/mL to 1000 ng/mL.
Trial Size

SARS-CoV Matrix Antibody

CATALOG NUMBER: 3527

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Rabbit
Species Reactivity:
Virus
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Virus
Homology:
Predicted reactivity based on immunogen sequence: SARS-CoV-2 Matrix protein: (identity 77%, homology 93%)
Immunogen:
Anti-SARS-CoV Matrix antibody (3527) was raised against a peptide corresponding to 13 amino acids near the amino-terminus of SARS-CoV Matrix protein.
The immunogen is located within the first 50 amino acids of SARS-CoV Matrix.
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
SARS-CoV Matrix antibody can be used for the detection of SARS-CoV Matrix protein in ELISA. It will detect 5 ng of free peptide at 1 μg/mL.
Isoforms:
SARS-CoV Matrix has one isoform (222aa).
Purification:
SARS-CoV Matrix Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SARS-CoV Matrix Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SARS-CoV Matrix antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
M
Additional Names:
SARS-CoV Matrix Antibody: Membrane protein, E1 glycoprotein, M protein
Protein Accession Number:
P59596
Protein Gi Number:
30173398
Ncbi Gene Id Number:
1489672
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019–20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. The membrane (M) protein or matrix protein is the most abundant structural protein and defines the shape of the viral envelope (3). It is an integral membrane protein involved in the budding of the viral particles and interacts with S (Spike) protein. It involves in organization of the nucleoprotein inside, which includes many copies of the N (nucleocapsid) protein bound to the genomic RNA. The M protein holds dominant cellular immunogenicity and has been determined as a protective antigen in humoral responses, which suggests it would serve as a potential target in vaccine design (4).
Background Reference 1:
Gorbalenya. bioRxiv: 2020.
Background Reference 2:
Hui et al. Int J Infect Dis. 2020;91:264-266.
Background Reference 3:
Neuman, et al. J Struct Biol. 2011;174(1):11–22.
Background Reference 4:
Liu et al. J Infect Dis. 2010;202(8):1171-80.
Citation 1:
Jaume, et al. Anti-severe acute respiratory syndrome coronavirus spike antibodies trigger infection of human immune cells via a pH- and cysteine protease-independent FcγR pathway. J Virol. 2011 Oct;85(20):10582-97. doi: 10.1128/JVI.00671-11. Epub 2011 Jul 20.PMID: 21775467
Citation 2:
Quijano-Rubio, et al. De novo design of modular and tunable allosteric biosensors. bioRxiv. 2020 Jul 20;2020.07.18.206946. doi: 10.1101/2020.07.18.206946.PMID: 32743576

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3527

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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