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<strong>Figure 1 ELISA Test </strong><br>Antibodies: SARS-CoV Spike Antibody, 3219 (1 μg/mL). A direct ELISA was performed using immunogen as coating antigen and the anti-SARS-CoV Spike antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.5 ng/mL to 1000ng/mL.
Figure 1 ELISA Test
Antibodies: SARS-CoV Spike Antibody, 3219 (1 μg/mL). A direct ELISA was performed using immunogen as coating antigen and the anti-SARS-CoV Spike antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.5 ng/mL to 1000ng/mL.
Trial Size

SARS-CoV Spike Antibody

CATALOG NUMBER: 3219

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Rabbit
Species Reactivity:
Virus
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Virus
Homology:
Predicted reactivity based on immunogen sequence: SARS-CoV2 Spike protein: (14%)
Immunogen:
Anti-SARS-CoV Spike antibody (3219) was raised against a peptide corresponding to 14 amino acids near the amino of SARS-CoV Spike glycoprotein.
The immunogen is located within the first 50 amino acids of SARS-CoV Spike.
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
SARS-CoV Spike antibody can be used for the detection of SARS-CoV Spike protein in ELISA. It will detect 5 ng of free peptide at 1 μg/mL.
Isoforms:
SARS-CoV Spike has one isoform (1255aa).
Purification:
SARS-CoV Spike Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SARS-CoV Spike Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SARS-CoV Spike antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
S
Additional Names:
SARS-CoV Spike Antibody: E2, Spike glycoprotein, E2, S glycoprotein
Protein Accession Number:
P59594
Protein Gi Number:
30173397
Ncbi Gene Id Number:
1489668
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SARS-CoV Spike Antibody: A novel coronavirus has been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Coronaviruses are a major cause of upper respiratory diseases in humans. The genomes of these viruses are positive-stranded RNA approximately 27-31kb in length. SARS infection can be mediated by the binding of the viral spike protein, a glycosylated 139 kDa protein and the major surface antigen of the virus, to the angiotensin-converting enzyme 2 (ACE2) on target cells. This binding can be blocked by a soluble form of ACE2.
Background Reference 1:
Marra et al. Science 2003;300:1399-404.
Background Reference 2:
Rota et al. Science 2003;300:1394-9.
Background Reference 3:
Navas-Nartin et al. J Neurovirol. 2004;10:75-85.
Background Reference 4:
Li et al. Nature 2003;426:450-4.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3219

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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