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<strong>Figure 1 ELISA Test </strong><br>Antibodies: SARS-CoV Spike Antibody, 3223 (1 μg/mL). A direct ELISA was performed using immunogen as coating antigen and the anti-SARS-CoV Spike antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 2 ng/mL to 1000 ng/mL.
Figure 1 ELISA Test
Antibodies: SARS-CoV Spike Antibody, 3223 (1 μg/mL). A direct ELISA was performed using immunogen as coating antigen and the anti-SARS-CoV Spike antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 2 ng/mL to 1000 ng/mL.
Trial Size

SARS-CoV Spike Antibody

CATALOG NUMBER: 3223

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Rabbit
Species Reactivity:
Virus
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Virus
Homology:
Predicted reactivity based on immunogen sequence: SARS-CoV2 Spike protein: (identity 65%, homology 88%)
Immunogen:
Anti-SARS-CoV Spike antibody (3223) was raised against a peptide corresponding to 17 amino acids near the center of SARS-CoV Spike glycoprotein.
The immunogen is located within amino acids 550-600 of SARS-CoV Spike.
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
SARS-CoV Spike antibody can be used for the detection of SARS-CoV Spike protein in ELISA. It will detect 2 ng of free peptide at 1 μg/mL.
Isoforms:
SARS-CoV Spike has one isoform (1255aa).
Purification:
SARS-CoV Spike Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SARS-CoV Spike Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SARS-CoV Spike antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
S
Additional Names:
SARS-CoV Spike antibody: E2, Spike glycoprotein, E2, S glycoprotein
Protein Accession Number:
P59594
Protein Gi Number:
30173397
Ncbi Gene Id Number:
1489668
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SARS Spike Antibody: A novel coronavirus has recently been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Coronaviruses are a major cause of upper respiratory diseases in humans. The genomes of these viruses are positive-stranded RNA approximately 27-31kb in length. SARS infection can be mediated by the binding of the viral spike protein, a glycosylated 139 kDa protein and the major surface antigen of the virus, to the angiotensin-converting enzyme 2 (ACE2) on target cells. This binding can be blocked by a soluble form of ACE2.
Background Reference 1:
Marra et al. Science 2003;300:1399-404.
Background Reference 2:
Rota et al. Science 2003;300:1394-9.
Background Reference 3:
Navas-Nartin et al. J Neurovirol. 2004;10:75-85.
Background Reference 4:
Li et al. Nature 2003;426:450-4.
Citation 1:
Clarke, et al. The iminosugars celgosivir, castanospermine and UV-4 inhibit SARS-CoV-2 replication. Glycobiology. 2020 Sep 26;cwaa091. doi: 10.1093/glycob/cwaa091.PMID: 32985653

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3223

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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