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Western blot analysis of extracts of various cell lines, using SART1 antibody (23-663) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunoprecipitation analysis of 150ug extracts of Jurkat cells using 3ug SART1 antibody (23-663). Western blot was performed from the immunoprecipitate using SART1 antibody (23-663) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using SART1 antibody (23-663) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

SART1 Antibody

CATALOG NUMBER: 23-663

Clonality:
Polyclonal
Tested Applications:
IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human SART1 (NP_005137.1).
Conjugate:
Unconjugated
Tested Applications:
IP, WB
Application Note:
WB: 1:500 - 1:2000
IP: 1:20 - 1:50
Positive Control 1:
HeLa
Positive Control 2:
Jurkat
Positive Control 3:
HepG2
Positive Control 4:
Mouse liver
Positive Control 5:
Mouse brain
Positive Control 6:
Mouse testis
Predicted Molecular Weight:
Observed: 120kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SART1
Additional Names:
SART1, squamous cell carcinoma antigen recognised by T cells, ARA1, Ara1, HOMS1, MGC2038, SART1259, Snu66, IgE autoantigen, SART1(259) protein, SART1(800) protein, U4/U6.U5 tri-snRNP-associated 110 kDa protein, squamous cell carcinoma antigen recognized by T cells 1, SNRNP110
Ncbi Gene Id Number:
9092
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full-length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor-rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri-snRNP to the pre-spliceosome in the spliceosome assembly pathway.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-663

List Size:
100 uL

List Price:

$436.00

Datasheet
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