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Western blot analysis of SATB1 in A20 cell lysate with SATB1 antibody at (A) 2 and (B) 4 μg/mL.
Immunohistochemistry of SATB1 in human brain with SATB1 antibody at 5 μg/mL.
Immunofluorescence of SATB1 in Human Brain cells with SATB1 antibody at 20 μg/mL.
Western blot analysis of SATB1 in A20 cell lysate with SATB1 antibody at (A) 2 and (B) 4 μg/mL.
Trial Size

SATB1 Antibody

CATALOG NUMBER: 4631

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
SATB1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of the human SATB1.
The immunogen is located within amino acids 620 - 670 of SATB1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SATB1 antibody can be used for detection of SATB1 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1288 - A20 Cell Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
SATB1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SATB1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SATB1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SATB1
Additional Names:
SATB1 Antibody: DNA-binding protein SATB1, Special AT-rich sequence-binding protein 1
Protein Accession Number:
Q01826
Protein Gi Number:
417747
Ncbi Gene Id Number:
6304
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SATB1 Antibody: Human special AT-rich sequence-binding protein-1 (SATB1) is a nuclear matrix/scaffold-associated region DNA-binding protein, predominantly expressed in the thymus and pre-B cells. Like its homolog SATB2, SATB1 selectively binds double-stranded, special AT-rich DNA sequences in which one strand exclusively consists of well-mixed A, T, and C nucleotides. SATB1 contains a dimerization domain that shares similarity with the PDZ motif, identified as an indispensable element for high-affinity binding of SATB1 to DNA. SATB1 constitutes a functional nuclear architecture that has a 'cage-like' protein distribution surrounding heterochromatin and regulates gene expression through chromatin remodeling/HDAC (histone deacetylase complex) and transcription factors recruitment. SATB1 functions as a 'genome organizer' essential for proper T-cell development. Recent studies show that SATB1 is necessary for breast cancer cells to become metastatic, and when ectopically expressed in non-metastatic cells, can induce invasive activity in vivo. At least two isoforms of SATB1 are known to exist.
Background Reference 1:
Dickinson LA and Kohwi-Shigematsu T. Nucleolin is a matrix attachment region DNA-binding protein that specifically recognizes a region with high base-unpairing potential. Mol. Cell. Biol.1995; 15:456-65.
Background Reference 2:
Szemes M, Gyorgy A, Pawaletz C, et al. Isolation and characterization of SATB2, a novel AT-rich DNA binding protein expressed in development- and cell-specific manner in the rat brain. Neurochem. Res.2006; 31:237-46.
Background Reference 3:
Purbey PK, Singh S, Kumar PP, et al. PDZ domain-mediated dimerization and homeodomain-directed specificity are required for high-affinity DNA binding by SATB1. Nucleic Acids Res.2008; 36:2107-22.
Background Reference 4:
Cai S, Han HJ and Kohwi-Shigematsu T. Tissue-specific nuclear architecture and gene expression regulated by SATB1. Nat. Genet.2003; 34:42-50.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4631

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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