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Western blot analysis of SCAMP4 in 3T3 cell lysate with SCAMP4 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of SCAMP4 in human brain tissue with SCAMP4 antibody at 2.5 μg/mL.
Immunofluorescence of SCAMP4 in Human Brain cells with SCAMP4 antibody at 20 μg/mL.
Western blot analysis of SCAMP4 in 3T3 cell lysate with SCAMP4 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

SCAMP4 Antibody

CATALOG NUMBER: 5289

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (93%)
Immunogen:
SCAMP4 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human SCAMP4.
The immunogen is located within the last 50 amino acids of SCAMP4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SCAMP4 antibody can be used for detection of SCAMP4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
SCAMP4 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SCAMP4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SCAMP4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SCAMP4
Additional Names:
SCAMP4 Antibody: SCAMP-4, Secretory carrier-associated membrane protein 4, Secretory carrier membrane protein 4
Protein Accession Number:
NP_524558
Protein Gi Number:
17738287
Ncbi Gene Id Number:
113178
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SCAMP4 Antibody: Secretory carrier membrane proteins (SCAMPs) are integral membrane proteins seen in secretory and endocytic carriers that are thought to play roles in membrane trafficking. One member of this family, SCAMP4, lacks the putative calcium binding and leucine zipper structures as well as the protein-protein interacting NPF domains common to most SCAMP family members. In rats, SCAMP4 mRNA is repressed by progesterone in brain regions associated with female sexual behavior. At least three isoforms of SCAMP4 are known to exist. This SCAMP4 antibody is predicted to not cross-react with any other member of the secretory carrier membrane protein family.
Background Reference 1:
Hubbard C, Singleton D, Rauch M, et al. The secretory carrier membrane protein family: structure and membrane topology. Mol. Biol. Cell2000; 11:2933-47.
Background Reference 2:
Krebs CJ and Pfaff DW. Expression of the SCAMP-4 gene, a new member of the secretory carrier membrane protein family, is repressed by progesterone in brain regions associated with female sexual behavior. Brain Res. Mol. Brain Res.2001; 88:144-54.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5289

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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