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To detect hsCD40-Ligand by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human sCD40-Ligand (XP-5263Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsCD40-Ligand.
To detect hsCD40-Ligand by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsCD40-Ligand is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hsCD40-Ligand by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsCD40-Ligand is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hsCD40-Ligand by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human sCD40-Ligand (XP-5263Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsCD40-Ligand.

sCD40 Ligand Antibody

CATALOG NUMBER: XP-5263

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hsCD40L (human sCD40 Ligand).
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA:
To detect hsCD40L by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hsCD40L.
Sandwich:
To detect hsCD40-Ligand by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human sCD40-Ligand (XP-5263Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsCD40-Ligand.
Western Blot:
To detect hsCD40L by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsCD40L is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Anti-hsCD40L specific antibody was purified by affinity chromatography employing immobilized hsCD40L matrix.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
sCD40 Ligand antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
CD40LG
Additional Names:
IGM, IMD3, TRAP, gp39, CD154, CD40L, HIGM1, T-BAM, TNFSF5, hCD40L, CD40 ligand, T-cell antigen Gp39, CD40-L
Protein Accession Number:
P29965
Protein Gi Number:
231718
Ncbi Gene Id Number:
959
User Note:
Centrifuge vial prior to opening.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5263

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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