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To detect hSCF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human SCF (XP-5265) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hSCF.
To detect hSCF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human SCF (XP-5265) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hSCF.

SCF Antibody (biotin)

CATALOG NUMBER: XP-5265Bt

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Biotin
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hSCF (human Stem Cell Factor).
Conjugate:
Biotin
Tested Applications:
ELISA, WB
Application Note:
ELISA:
Sandwich:
To detect hSCF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human SCF (XP-5265) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hSCF.
Western Blot:
To detect hSCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Anti-hSCF specific antibody was purified by affinity chromatography and then biotinylated.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
SCF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
KITLG
Additional Names:
SF, MGF, SCF, FPH2, KL-1, Kitl, SHEP7, Kit ligand, Mast cell growth factor
Protein Accession Number:
P21583
Protein Gi Number:
134289
Ncbi Gene Id Number:
4254
User Note:
Centrifuge vial prior to opening.
Background:
Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of mast cells and is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It also mediates cell to cell adhesion and acts synergistically with other cytokines, probably interleukins. A soluble form is produced by proteolytic processing of isoform 1 in the extracellular domain.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5265Bt

List Size:
0.05 mg

List Price:

$408.00

Datasheet
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