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To detect mSCF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Murine SCF (XP-5266) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mSCF.
To detect mSCF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Murine SCF (XP-5266) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mSCF.

SCF Antibody (biotin)

CATALOG NUMBER: XP-5266Bt

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Biotin
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mSCF (murine SCF).
Conjugate:
Biotin
Tested Applications:
ELISA, WB
Application Note:
ELISA:
Sandwich:
To detect mSCF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine SCF (XP-5266) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSCF.
Western Blot:
To detect mSCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Anti-mSCF specific antibody was purified by affinity chromatography and then biotinylated.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
SCF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
Kitl
Additional Names:
Gb, SF, Sl, Clo, Con, Mgf, SCF, SLF, blz, Kitlg, contrasted, Kitl, Slf, Kit ligand, Hematopoietic growth factor KL, MGF
Protein Accession Number:
P20826
Protein Gi Number:
134290
Ncbi Gene Id Number:
17311
User Note:
Centrifuge vial prior to opening.
Background:
Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of mast cells and is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It also mediates cell to cell adhesion and acts synergistically with other cytokines, probably interleukins. A soluble form is produced by proteolytic processing of isoform 1 in the extracellular domain.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5266Bt

List Size:
0.05 mg

List Price:

$408.00

Datasheet
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