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Western blot analysis of SCFD1 antibody in MCF-7 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with SCFD1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of MDA-MB468 cells using SCFD1 Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of SCFD1 antibody in MCF-7 cell line lysates (35ug/lane)

SCFD1 Antibody

CATALOG NUMBER: 62-391

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This SCFD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 543-572 amino acids from the C-terminal region of human SCFD1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
72 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SCFD1
Additional Names:
Sec1 family domain-containing protein 1, SLY1 homolog, Sly1p, Syntaxin-binding protein 1-like 2, SCFD1, C14orf163, KIAA0917, STXBP1L2
Protein Accession Number:
Q8WVM8
Protein Gi Number:
51316882
Ncbi Gene Id Number:
23256
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SCFD1 is involved in vesicular transport between the endoplasmic reticulum and the Golgi.
Background Reference 1:
Matsuo,N., J. Biol. Chem. 272 (26), 16438-16444 (1997)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-391

List Size:
400 ul

List Price:

$495.00

Datasheet
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