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Western blot analysis of extracts of various cell lines, using SCG3 antibody (23-192) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human stomach using SCG3 antibody (23-192) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using SCG3 antibody (23-192) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using SCG3 antibody (23-192) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using SCG3 antibody (23-192) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

SCG3 Antibody

CATALOG NUMBER: 23-192

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 199-468 of human SCG3 (NP_037375.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
22Rv1
Positive Control 2:
HepG2
Positive Control 3:
Mouse brain
Positive Control 4:
Mouse lung
Positive Control 5:
Mouse heart
Positive Control 6:
Rat brain
Predicted Molecular Weight:
Observed: 53kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SCG3
Additional Names:
Secretogranin-3, Secretogranin III, SgIII, SCG3
Ncbi Gene Id Number:
29106
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. Granins may serve as precursors for biologically active peptides. Some granins have been shown to function as helper proteins in sorting and proteolytic processing of prohormones; however, the function of this protein is unknown. Two transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-192

List Size:
100 uL

List Price:

$436.00

Datasheet
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