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Western blot analysis of extracts of various cell lines, using SCN3A antibody (13-412) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.
Western blot analysis of extracts of various cell lines, using SCN3A antibody (13-412) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.

SCN3A Antibody

CATALOG NUMBER: 13-412

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 274-400 of human SCN3A (NP_008853.3).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:1000
Positive Control 1:
293T
Positive Control 2:
HT-1080
Predicted Molecular Weight:
Observed: 350kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SCN3A
Additional Names:
EIEE62, FFEVF4, NAC3, Nav1.3, sodium channel protein type 3 subunit alpha, brain III voltage-gated sodium channel, sodium channel protein brain III subunit alpha, sodium channel protein type III subunit alpha, sodium channel, voltage gated, type III alpha subunit, sodium channel, voltage-gated, type III, alpha polypeptide, voltage-gated sodium channel subtype III, voltage-gated sodium channel subunit alpha Nav1.3
Ncbi Gene Id Number:
6328
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Voltage-gated sodium channels are transmembrane glycoprotein complexes composed of a large alpha subunit with 24 transmembrane domains and one or more regulatory beta subunits. They are responsible for the generation and propagation of action potentials in neurons and muscle. This gene encodes one member of the sodium channel alpha subunit gene family, and is found in a cluster of five alpha subunit genes on chromosome 2. Multiple transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-412

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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