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Western blot analysis of extracts of various cell lines, using SCNN1A antibody (19-716) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of human lung cancer cells using SCNN1A antibody (19-716) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat lung cells using SCNN1A antibody (19-716) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse kidney cells using SCNN1A antibody (19-716) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of human lung cancer cells using SCNN1A antibody (19-716) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat lung cells using SCNN1A antibody (19-716) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using SCNN1A antibody (19-716) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

SCNN1A Antibody

CATALOG NUMBER: 19-716

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 105-225 of human SCNN1A (NP_001029.1).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:200 - 1:2000
IF: 1:50 - 1:200
Positive Control 1:
Mouse lung
Positive Control 2:
Mouse intestine
Predicted Molecular Weight:
Observed: 76kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SCNN1A
Additional Names:
Amiloride-sensitive sodium channel subunit alpha, Alpha-NaCH, Epithelial Na(+) channel subunit alpha, Alpha-ENaC, ENaCA, Nonvoltage-gated sodium channel 1 subunit alpha, SCNEA, SCNN1A, SCNN1
Ncbi Gene Id Number:
6337
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the alpha subunit, and mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1), a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-716

List Size:
100 uL

List Price:

$419.00

Out of stock

Datasheet
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