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Western Blot at 1:2000 dilution + Daudi whole cell lysate Lysates/proteins at 20 ug per lane.
Overlay histogram showing WiDr cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with SCNN1A Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Confocal immunofluorescent analysis of SCNN1A Antibody with Hela cell followed by Alexa Fluor489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:2000 dilution + Daudi whole cell lysate Lysates/proteins at 20 ug per lane.

SCNN1A Antibody

CATALOG NUMBER: 63-651

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This SCNN1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 365-391 amino acids from the Central region of human SCNN1A.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:2000
For FACS starting dilution is: 1:25
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
76 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SCNN1A
Additional Names:
Amiloride-sensitive sodium channel subunit alpha, Alpha-NaCH, Epithelial Na(+) channel subunit alpha, Alpha-ENaC, ENaCA, Nonvoltage-gated sodium channel 1 subunit alpha, SCNEA, SCNN1A, SCNN1
Protein Accession Number:
P37088
Protein Gi Number:
585966
Ncbi Gene Id Number:
6337
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the alpha subunit, and mutations in this gene have been sociated with pseudohypoaldosteronism type 1 (PHA1), a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids.
Background Reference 1:
Azad A.K., et.al., Hum. Mutat. 30:1093-1103(2009).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-651

List Size:
400 ul

List Price:

$495.00

Datasheet
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