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Western blot analysis of extracts of various cell lines, using SCNN1G antibody (22-017) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.
Immunohistochemistry of paraffin-embedded mouse lung using SCNN1G antibody (22-017) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using SCNN1G antibody (22-017) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.

SCNN1G Antibody

CATALOG NUMBER: 22-017

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 85-355 of human SCNN1G (NP_001030.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:1000
IHC: 1:50 - 1:100
Positive Control 1:
SH-SY5Y
Positive Control 2:
Mouse kidney
Predicted Molecular Weight:
Observed: 80kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SCNN1G
Additional Names:
SCNN1G, sodium channel, nonvoltage-gated 1, gamma, ENaCg, ENaCgamma, PHA1, SCNEG, ENaC gamma subunit, amiloride-sensitive epithelial sodium channel gamma subunit, BESC3
Ncbi Gene Id Number:
6340
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the gamma subunit, and mutations in this gene have been associated with Liddle syndrome.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-017

List Size:
100 uL

List Price:

$436.00

Datasheet
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