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Western blot analysis of SCP2 Antibody in A2058 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with SCP2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of HepG2 cells using SCP2 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of SCP2 Antibody in A2058 cell line lysates (35ug/lane)

SCP2 Antibody

CATALOG NUMBER: 63-564

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rabbit, Rat
Immunogen:
This SCP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 358-385 amino acids from the Central region of human SCP2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
59 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SCP2
Additional Names:
Non-specific lipid-transfer protein, NSL-TP, Propanoyl-CoA C-acyltransferase, SCP-chi, SCPX, Sterol carrier protein 2, SCP-2, Sterol carrier protein X, SCP-X, SCP2
Protein Accession Number:
P22307
Protein Gi Number:
2507456
Ncbi Gene Id Number:
6342
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SCP2 mediates in vitro the transfer of all common phospholipids, cholesterol and gangliosides between membranes. It may play a role in regulating steroidogenesis.
Background Reference 1:
Wu, Y.B. et al.Biol Chem. 2009 January 2; 284(1): 640?48.
Background Reference 2:
Baker,M.E., et.al., DNA Cell Biol. 10 (9), 695-698 (1991)
Background Reference 3:
Vila,A., et.al., Biochemistry 43 (39), 12592-12605 (2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-564

List Size:
400 ul

List Price:

$495.00

Datasheet
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