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Western blot analysis of SCRN2 in 293 cell tissue lysate with SCRN2 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunocytochemistry of SCRN2 in 293 cells with SCRN2 antibody at 20 μg/mL.
Immunofluorescence of SCRN2 in 293 cells with SCRN2 antibody at 20 μg/mL.
Western blot analysis of SCRN2 in 293 cell tissue lysate with SCRN2 antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

SCRN2 Antibody

CATALOG NUMBER: 5851

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
SCRN2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SCRN2.
The immunogen is located within amino acids 320 - 370 of SCRN2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
SCRN2 antibody can be used for detection of SCRN2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Positive Control 2:
Cat. No. 17-010 - HEK293 Cell Slide
Purification:
SCRN2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SCRN2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SCRN2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SCRN2
Additional Names:
SCRN2 Antibody: Ses2, Secernin-2
Protein Accession Number:
EAW94970
Protein Gi Number:
119615376
Ncbi Gene Id Number:
90507
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SCRN2 Antibody: SCRN2 is a member of the secernin family of proteins which includes the homologous SCRN1, a protein that was first identified as being involved in the regulation of exocytosis from peritoneal mast cells. Little is known of SCRN2, but studies have shown that SCRN1 may possess epitopes that function as tumor-associated antigens in gastric cancers and increased SCRN1 expression in patients with colorectal cancer correlated with poor prognosis, suggesting that SCRN2 may also be involved in protein secretion or the regulation of cell growth.
Background Reference 1:
Way G, Morrice N, Smythe C, et al. Purification and identification of Secernin, a novel cytosolic protein that regulates exocytosis in mast cells. Mol. Biol. Cell2002; 13:3344-54.
Background Reference 2:
Suda T, Tsunoda T, Uchida N, et al. Identification of secernin 1 as a novel immunotherapy target for gastric cancer using the expression profiles of cDNA microarray. Cancer Sci.2006; 97:411-9.
Background Reference 3:
Miyoshi N, Ishii H, Mimori K, et al. SCRN1 is a novel marker for prognosis in colorectal cancer. J. Surg. Oncol.2010; 101:156-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5851

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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