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Western blot analysis of SCRN3 in human breast tissue lysate with SCRN3 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of SCRN3 in 293 cells with SCRN3 antibody at 20 μg/mL.
Immunofluorescence of SCRN3 in 293 cells with SCRN3 antibody at 20 μg/mL.
Western blot analysis of SCRN3 in human breast tissue lysate with SCRN3 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

SCRN3 Antibody

CATALOG NUMBER: 5853

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
SCRN3 antibody was raised against a 15 amino acid synthetic peptide near the center of human SCRN3.
The immunogen is located within amino acids 230 - 280 of SCRN3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
SCRN3 antibody can be used for detection of SCRN3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1311 - Human Breast Tissue Lysate
Positive Control 2:
Cat. No. 17-010 - HEK293 Cell Slide
Purification:
SCRN3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SCRN3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SCRN3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SCRN3
Additional Names:
SCRN3 Antibody: SES3, Secernin-3
Protein Accession Number:
AAI19685
Protein Gi Number:
111601409
Ncbi Gene Id Number:
79634
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SCRN3 Antibody: SCRN3 is a member of the secernin family of proteins which includes the homologous SCRN1, a protein that was first identified as being involved in the regulation of exocytosis from peritoneal mast cells. Little is known of SCRN3, but studies have shown that SCRN1 may possess epitopes that function as tumor-associated antigens in gastric cancers and increased SCRN1 expression in patients with colorectal cancer correlated with poor prognosis, suggesting that SCRN3 may also be involved in protein secretion or the regulation of cell growth.
Background Reference 1:
Way G, Morrice N, Smythe C, et al. Purification and identification of Secernin, a novel cytosolic protein that regulates exocytosis in mast cells. Mol. Biol. Cell2002; 13:3344-54.
Background Reference 2:
Suda T, Tsunoda T, Uchida N, et al. Identification of secernin 1 as a novel immunotherapy target for gastric cancer using the expression profiles of cDNA microarray. Cancer Sci.2006; 97:411-9.
Background Reference 3:
Miyoshi N, Ishii H, Mimori K, et al. SCRN1 is a novel marker for prognosis in colorectal cancer. J. Surg. Oncol.2010; 101:156-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5853

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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