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Western blot analysis of SCUBE1 in Daudi cell lysate with SCUBE1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of SCUBE1 in Daudi cells with SCUBE1 antibody at 20 μg/mL.
Immunofluorescence of SCUBE1 in Daudi cells with SCUBE1 antibody at 20 μg/mL.
Western blot analysis of SCUBE1 in Daudi cell lysate with SCUBE1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

SCUBE1 Antibody

CATALOG NUMBER: 5843

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (93%)
Immunogen:
SCUBE1 antibody was raised against a 14 amino acid synthetic peptide near the center of human SCUBE1.
The immunogen is located within amino acids 460 - 510 of SCUBE1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
SCUBE1 antibody can be used for detection of SCUBE1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1224 - Daudi Cell Lysate
Purification:
SCUBE1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SCUBE1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SCUBE1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SCUBE1
Additional Names:
SCUBE1 Antibody: Signal peptide, CUB and EGF-like domain-containing protein 1
Protein Accession Number:
NP_766638
Protein Gi Number:
120587029
Ncbi Gene Id Number:
80274
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SCUBE1 Antibody: SCUBE1 is an EGF-related protein with a CUB (C1s-like) domain that is expressed primarily in the developing gonad, nervous system, somites, surface ectoderm and limb buds. The N-terminal EGF-like repeats of SCUBE1 participate in the formation of adhesions in a Ca2+-dependent fashion. In contrast, the C-terminal cysteine-rich motifs and CUB domain can directly bind and antagonize bone morphogenic protein activity; genetic ablation of this region resulted in brain malformation in mice embryos, suggesting SCUBE1 plays a role in brain morphogenesis. SCUBE1 has also been implicated in early craniofacial development in mice.
Background Reference 1:
Grimmond S, Larder R, Van Hateren N, et al. Cloning, mapping, and expression analysis of a gene encoding a novel mammalian EGF-related protein (SCUBE1). Genomics2000; 70:74-81.
Background Reference 2:
Tu C, Yan Y, Wu S, et al. Domain and function analysis of a novel platelet-endothelial cell surface protein, SCUBE1. J. Biol. Chem.2008; 283:12478-88.
Background Reference 3:
Xaiver GM, Sharpe PT, and Colbourne MT. Scube1 is expressed during facial development in the mouse. J. Exp. Zool. B. Mol. Dev. Evol.2009; 312B:518-24.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5843

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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