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Western blot analysis of SCUBE2 in Daudi cell lysate with SCUBE2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of SCUBE2 in Daudi cells with SCUBE2 antibody at 20 μg/mL.
Immunofluorescence of SCUBE2 in Daudi cells with SCUBE2 antibody at 20 μg/mL.
Western blot analysis of SCUBE2 in Daudi cell lysate with SCUBE2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

SCUBE2 Antibody

CATALOG NUMBER: 5845

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Immunogen:
SCUBE2 antibody was raised against a 17 amino acid synthetic peptide near the center of human SCUBE2.
The immunogen is located within amino acids 480 - 530 of SCUBE2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
SCUBE2 antibody can be used for detection of SCUBE2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1224 - Daudi Cell Lysate
Purification:
SCUBE2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SCUBE2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SCUBE2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SCUBE2
Additional Names:
SCUBE2 Antibody: CEGB1, CEGF1, CEGP1, Signal peptide, CUB and EGF-like domain-containing protein 2, Protein CEGP1
Protein Accession Number:
NP_066025
Protein Gi Number:
283046663
Ncbi Gene Id Number:
57758
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SCUBE2 Antibody: SCUBE2 is a member of a family of secreted glycoproteins that contain N-terminal EGF-like repeats and C-terminal cysteine-rich motifs and CUB domain and are expressed in embryonic neuroectoderm and vascular epithelium. SCUBE2 is a close homolog to SCUBE1 and can form a heterodimeric complex with SCUBE1. SCUBE2 is thought to participate in the Hedgehog signaling pathway by specifically interacting with Sonic Hedgehog (SHH) and the receptor PTCH1, enhancing the SHH signaling activity in the raft microdomains of the plasma membrane, and may thus play important roles in organ development and tumor progression.
Background Reference 1:
Grimmond S, Larder R, Van Hateren N, et al. Cloning, mapping, and expression analysis of a gene encoding a novel mammalian EGF-related protein (SCUBE1). Genomics2000; 70:74-81.
Background Reference 2:
Yang RB, Ng CK, Wasserman SM, et al. Identification of a novel family of cell-surface proteins expressed in human vascular endothelium. J. Biol. Chem.2002; 277:46364-73.
Background Reference 3:
Grimmond S, Larder R, Van Hateren N, et al. Cloning, mapping, and expression analysis of a gene encoding a novel mammalian EGF-related protein (SCUBE1). Genomics2000; 70:74-81.
Background Reference 4:
Tsai M, Cheng C, Lin Y, et al. Isolation and characterization of a secreted, cell-surface glycoprotein SCUBE2 from humans. Biochem. J. 2009; 422:119-28.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5845

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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