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Western blot analysis of SCUBE3 in mouse kidney tissue lysate with SCUBE3 antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of SCUBE3 in human kidney tissue with SCUBE3 antibody at 20 μg/mL.
Western blot analysis of SCUBE3 in mouse kidney tissue lysate with SCUBE3 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

SCUBE3 Antibody

CATALOG NUMBER: 7003

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
SCUBE3 antibody was raised against a 15 amino acid synthetic peptide near the center of human SCUBE3.
The immunogen is located within amino acids 210 - 260 of SCUBE3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
SCUBE3 antibody can be used for detection of SCUBE3 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least two isoforms of SCUBE3 are known to exist. SCUBE3 antibody is predicted to not cross-react with other SCUBE members
Positive Control 1:
Cat. No. 1405 - Mouse Kidney Tissue Lysate
Positive Control 2:
Cat. No. 10-401 - Human Kidney Tissue Slide
Predicted Molecular Weight:
109 kDa
Purification:
SCUBE3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SCUBE3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SCUBE3 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SCUBE3
Additional Names:
SCUBE3 Antibody: CEGF3, CEGF3, Signal peptide, CUB and EGF-like domain-containing protein 3
Protein Accession Number:
NP_689966
Protein Gi Number:
31377568
Ncbi Gene Id Number:
222663
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SCUBE3 Antibody: SCUBE3 is a member of a family of secreted glycoproteins that contain N-terminal EGF-like repeats and C-terminal cysteine-rich motifs and CUB domain and is highly expressed in primary osteoblasts and bones, and to a lesser extent in heart. Other studies have shown that overexpression of SCUBE3 in mice induced cardiac hypertrophy, suggesting that it may also play a role in the regulation of cardiac growth.. SCUBE3 has been shown to be an endogenous TGF-β receptor ligand and is thought to promote lung cancer cell mobility and invasiveness. In lung cancer cells, the secreted SCUBE3 protein was cleaved by MMP2 and MMP9, allowing the activation of the TGF-β receptor, the increase of Smad2/3 transcriptional activity and the upregulation of expression of proteins such as TGF-β1, VEGF, Snail, and Slug.
Background Reference 1:
Wu BT, Su YH, Tsai MT, et al. A novel secreted, cell-surface glycoprotein containing multiple epidermal growth factor-like repeats and one CUB domain is highly expressed in primary osteoblasts and bones. J. Biol. Chem. 2004; 279:37485-90.
Background Reference 2:
Yang RB, Ng CK, Wasserman SM, et al. Identification of a novel family of cell-surface proteins expressed in human vascular endothelium. J. Biol. Chem. 2002; 277:46364-73.
Background Reference 3:
Yang HY, Cheng CF, Djoko B, et al. Transgenic overexpression of the secreted, extracellular EGF-CUB domain containing protein SCUBE3 induces cardiac hypertrophy in mice. Cardiovas. Res. 2007; 75:139-47.
Background Reference 4:
Wu YY, Peck K, Chang YL, et al. SCUBE3 is an endogenous TGF-beta receptor ligand and regulates the epithelial-mesenchymal transition in lung cancer. Oncogene 2011; 30:3682-93.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7003

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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