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Antibody used in WB on Human Jurkat cells at 0.2-1 ug/ml.
Antibody used in WB on Human Jurkat cells at 0.2-1 ug/ml.

SCYE1 Antibody

CATALOG NUMBER: 29-340

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SCYE1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
SCYE1 antibody can be used for detection of SCYE1 by ELISA at 1:62500. SCYE1 antibody can be used for detection of SCYE1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
34 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store SCYE1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
AIMP1
Additional Names:
SCYE1, p43, HLD3, EMAP2, SCYE1, EMAPII
Protein Accession Number:
NP_004748
Protein Gi Number:
45006986
Ncbi Gene Id Number:
9255
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SCYE1 is a cytokine that is specifically induced by apoptosis. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor of SCYE1 (pro-SCYE1) is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex. Therefore, pro-SCYE1 may function in binding RNA as part of the tRNA synthetase complex in normal cells and in stimulating inflammatory responses after proteolytic cleavage in tumor cells.The protein encoded by this gene is a cytokine that is specifically induced by apoptosis. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor of SCYE1 (pro-SCYE1) is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex. Therefore, pro-SCYE1 may function in binding RNA as part of the tRNA synthetase complex in normal cells and in stimulating inflammatory responses after proteolytic cleavage in tumor cells.
Background Reference 1:
Tandle, A.T., (2005) Cytokine 30 (6), 347-358.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

29-340

List Size:
100 ul

List Price:

$519.00

Datasheet
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