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Western blot analysis in human placenta tissue lysates (35ug/lane).
SCYL1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in human placenta tissue lysates (35ug/lane).

SCYL1 Antibody

CATALOG NUMBER: 62-735

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This SCYL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-185 amino acids from the N-terminal region of human SCYL1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
90 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SCYL1
Additional Names:
N-terminal kinase-like protein, Coated vesicle-associated kinase of 90 kDa, SCY1-like protein 1, Telomerase regulation-associated protein, Telomerase transcriptional element-interacting factor, Teratoma-associated tyrosine kinase, SCYL1, CVAK90, GKLP, NTKL, TAPK, TEIF, TRAP
Protein Accession Number:
Q96KG9
Protein Gi Number:
74762671
Ncbi Gene Id Number:
57410
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SCYL1 forms multimers following transfection into COS-7 cells. SCYL1 forms a 300-kD trimer using crosslinking reagents. Biochemical analysis revealed no phosphorylation or autophosphorylation activity.The 707-amino acid SCYL1 variant, variant 2, localized to centrosomes during mitosis. During interphase, fluorescence-tagged variant 2 localized in the cytoplasm as well as centrosomes. However, at the beginning of mitosis, the fluorescence appeared as a pair of bright nuclear foci that followed centrosome localization throughout mitosis, while maintaining diffuse cytoplasmic labeling. Endogenous variant 2 in HeLa cells showed a similar staining pattern. Centrosomal localization was independent of microtubules.
Background Reference 1:
Tang, Z., et al., Biochem. Biophys. Res. Commun. 324(4):1324-1332 (2004).
Background Reference 2:
Kato, M., et al., Genomics 79(6):760-767 (2002).
Background Reference 3:
Liu, S.C., et al., Biochim. Biophys. Acta 1517(1):148-152 (2000).
Background Reference 4:
van Asseldonk, M., et al., Genomics 66(1):35-42 (2000).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-735

List Size:
400 ul

List Price:

$495.00

Datasheet
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