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Western blot analysis of anti-SCYL2 Pab (AP7024b) in T47D (left) and mouse brain (right) tissue lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human brain tissue reacted with SCYL2 , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-SCYL2 Pab (AP7024b) in T47D (left) and mouse brain (right) tissue lysates (35ug/lane).

SCYL2 Antibody

CATALOG NUMBER: 62-598

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This SCYL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 643-672 amino acids from the C-terminal region of human SCYL2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
104 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SCYL2
Additional Names:
SCY1-like protein 2, Coated vesicle-associated kinase of 104 kDa, SCYL2, CVAK104, KIAA1360
Protein Accession Number:
Q6P3W7
Protein Gi Number:
74762350
Ncbi Gene Id Number:
55681
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SCYL2 is a component of AP2-containing clathrin coated structures at the plasma membrane or of endocytic coated vesicles. SCYL2 is a probable serine/threonine-protein kinase that phosphorylates, in vitro, the beta2-subunit of the plasma membrane adapter complex AP2 and other proteins in presence of poly-L-lysine. SCYL2 may also regulate clathrin-dependent trafficking between the TGN and/or the endosomal system.
Background Reference 1:
Ota, T., et al., Nat. Genet. 36(1):40-45 (2004).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-598

List Size:
400 ul

List Price:

$495.00

Datasheet
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