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To detect Human SDF-1-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human SDF-1-alpha (XP-5269Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human SDF-1-alpha.
To detect Human SDF-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human SDF-1-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Human SDF-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human SDF-1-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained formalin-fixed, paraffin-embedded sections of human colorectal adenocarcinoma. The recommended concentration is 0.5 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
To detect Human SDF-1-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human SDF-1-alpha (XP-5269Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human SDF-1-alpha.

SDF-1a Antibody

CATALOG NUMBER: XP-5269

Clonality:
Polyclonal
Tested Applications:
ELISA, Neutralization Assay, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hSDF-1-alpha (human Stromal Cell-Derived Factor-1-alpha).
Conjugate:
Unconjugated
Tested Applications:
ELISA, Neutralization Assay, WB
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hSDF1α (100 ng/mL), a concentration of 2-4 μg/mL of this antibody is required.
ELISA:
To detect hSDF-1-alpha by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hSDF-1-alpha.
Sandwich:
To detect hSDF-1α by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human SDF-1α (XP-5269Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hSDF-1α.
Western Blot:
To detect hSDF-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSDF-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Anti-hSDF-1-alpha specific antibody was purified by affinity chromatography employing immobilized hSDF-1-alpha matrix.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
SDF-1a antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
CXCL12
Additional Names:
IRH, PBSF, SDF1, TLSF, TPAR1, SCYB12, SDF1A, SDF1B, Stromal cell-derived factor 1, C-X-C motif chemokine 12, SDF-1
Protein Accession Number:
P48061
Protein Gi Number:
1352728
Ncbi Gene Id Number:
6387
User Note:
Centrifuge vial prior to opening.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5269

List Size:
0.1 mg

List Price:

$408.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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