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Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using SDHA Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Western blot analysis in Hela and mouse C2C12 cell line lysates (35ug/lane).
Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using SDHA Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

SDHA Antibody

CATALOG NUMBER: 60-990

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Xenopus, Rat
Immunogen:
This SDHA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 577-605 amino acids from the C-terminal region of human SDHA.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:25
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
73 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SDHA
Additional Names:
Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial, Flavoprotein subunit of complex II, Fp, SDHA, SDH2, SDHF
Protein Accession Number:
P31040
Protein Gi Number:
1169337
Ncbi Gene Id Number:
6389
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Can act as a tumor suppressor.
Background Reference 1:
Hirawake H., et al. J. Biochem. 116:221-227(1994).
Background Reference 2:
Morris A.A.M., et al. Biochim. Biophys. Acta 1185:125-128(1994).
Background Reference 3:
Parfait B., et al. Hum. Genet. 106:236-243(2000).
Background Reference 4:
Ota T., et al. Nat. Genet. 36:40-45(2004).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-990

List Size:
400 ul

List Price:

$495.00

Datasheet
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