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Western blot analysis of SDHAF1 in 3T3 cell lysate with SDHAF1 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of SDHAF1 in 3T3 cells with SDHAF1 antibody at 5 μg/mL.
Immunofluorescence of SDHAF1 in 3T3 cells with SDHAF1 antibody at 20 μg/mL.
Western blot analysis of SDHAF1 in 3T3 cell lysate with SDHAF1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

SDHAF1 Antibody

CATALOG NUMBER: 6879

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
SDHAF1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SDHAF1.
The immunogen is located within the last 50 amino acids of SDHAF1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
SDHAF1 antibody can be used for detection of SDHAF1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
SDHAF1 antibody is predicted to not cross-react with other SDHAF protein family members.
Positive Control 1:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Positive Control 2:
Cat. No. 17-201 - 3T3/BALB Cell Slide
Purification:
SDHAF1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SDHAF1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SDHAF1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SDHAF1
Additional Names:
SDHAF1 Antibody: LYRM8, LYRM8, Succinate dehydrogenase assembly factor 1, mitochondrial, LYR motif-containing protein 8, SDH assembly factor 1
Protein Accession Number:
NP_001036096
Protein Gi Number:
111038124
Ncbi Gene Id Number:
644096
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SDHAF1 Antibody: SDHAF1 (Succinate dehydrogenase complex assembly factor 1) is one of the subunits of the succinate dehydrogenase (SDH) complex (or complex II) of the mitochondrial respiratory chain. SDHAF1 resides in the mitochondria, and is essential for SDH assembly, but does not physically associate with the complex in vivo. Mutations in this gene are associated with SDH-defective infantile leukoencephalopathy (mitochondrial complex II deficiency). Unlike the related protein SDHAF2, SDHAF1 is not thought to be a tumor suppressor.
Background Reference 1:
Ghezzi D, Goffrini P, Uziel G, et al. SDHAF1, encoding a LYR complex-II specific assembly factor, is mutated in SDH-defective infantile leukoencephalopathy. Nat. Genet. 2009; 41:654-6.
Background Reference 2:
Hensen EE and Bayley JP. Recent advances in the genetics of SDH-related paraganglioma and pheochromocytoma. Fam. Cancer 2011; 10:355-63.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6879

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

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