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Western blot analysis of SDHD in EL4 cell lysate with SDHD antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of SDHD in EL4 cells with SDHD antibody at 2.5 μg/mL.
Immunofluorescence of SDHD in EL4 cells with SDHD antibody at 20 μg/mL.
Western blot analysis of SDHD in EL4 cell lysate with SDHD antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

SDHD Antibody

CATALOG NUMBER: 6847

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Sheep: (100%), Pig: (80%)
Immunogen:
SDHD antibody was raised against a 15 amino acid synthetic peptide near the center of human SDHD.
The immunogen is located within amino acids 30 - 80 of SDHD.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
SDHD antibody can be used for detection of SDHD by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
At least four isoforms of SDHD are known to exist; this antibody will detect the two longest isoforms.
Positive Control 1:
Cat. No. 1287 - EL4 Cell Lysate
Positive Control 2:
Cat. No. 17-207 - EL4 Cell Slide
Purification:
SDHD Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SDHD Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SDHD antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SDHD
Additional Names:
SDHD Antibody: TRAP1, TRAP-1, SDH4, CII-4, CybS
Protein Accession Number:
NP_002993
Protein Gi Number:
4506865
Ncbi Gene Id Number:
9392
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SDHD Antibody: The mitochondrial succinate dehydrogenase complex subunit D (SDHD) is one of four proteins that make up the tricarboxylic cycle enzyme succinate dehydrogenase (SCH). Studies have shown that mutations in SDHD often leads to hereditary paragangliomas, usually benign tumors of the autonomic nervous system, suggesting that SDHD also plays a role as a tumor-suppressor gene. In one family with a nonsense mutation (R22X) in the SDHD gene, a loss of heterozygosity was found in the paragangliomas, and within these tumors the enzymatic activity of Complex II in the mitochondrial respiratory chain was completely abolished. Furthermore, high levels of angiogenic factors EPAS1 and VEGF was observed, which may stimulate tumor growth.
Background Reference 1:
Baysal BE, Ferrell RE, Willett-Brozick JE, et al. Mutations in SDHD, a mitochondrial complex II gene, in hereditary paraganglioma. Science 2000; 287:848-51.
Background Reference 2:
Saraste M. Oxidative phosphorylation at the fin de siecle. Science 1999; 283:1488-93.
Background Reference 3:
Knudson AG. Genetics of human cancer. Annu. Rev. Genet. 1986; 20:231-51.
Background Reference 4:
Gimenez-Roqueplo AP, Favier J, Rustin P, et al. The R22X mutation of the SDHD gene in hereditary paraganglioma abolishes the enzymatic activity of Complex II in the mitochondrial respiratory chain and activates the hypoxia pathway. Am. J. Hum. Genet. 2001; 69:1186-97.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6847

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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