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ELISA results using Seasonal H1N1 Hemagglutinin antibody at 1 μg/mL and the blocking and corresponding peptides at 50, 10, 2 and 0 ng/mL.
ELISA results using Seasonal H1N1 Hemagglutinin antibody at 1 μg/mL and the blocking and corresponding peptides at 50, 10, 2 and 0 ng/mL.
Trial Size

Seasonal H1N1 Hemagglutinin Antibody

CATALOG NUMBER: 5235

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Rabbit
Species Reactivity:
Virus
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Virus
Immunogen:
Hemagglutinin antibody was raised against a synthetic peptide from the Hemagglutinin protein.
The peptide sequence is unique from the peptide sequence for product 5231 and 5239.
This antibody is a cognate pair with antibody 5237.
The immunogen is located within amino acids 180 - 230 of Seasonal H1N1 Hemagglutinin.
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Hemagglutinin antibody can be used for the detection of the Hemagglutinin protein from the H1N1 strain of common influenza A in ELISA. It will detect 2 ng of free peptide at 1 μg/mL.
Specificity:
This antibody is specific for the seasonal H1N1 influenza Hemagglutinin and will not recognize the corresponding Hemagglutinin sequence from the swine-origin H1N1 influenza (A/California/14/2009 (H1N1)). Will not cross-react with peptide corresponding to the swine-origin H1N1 influenza Hemagglutinin.
Purification:
Seasonal H1N1 Hemagglutinin Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Seasonal H1N1 Hemagglutinin Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Seasonal H1N1 Hemagglutinin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HA
Additional Names:
Seasonal H1N1 Hemagglutinin Antibody:
Protein Accession Number:
ACA28844
Protein Gi Number:
168805691
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Seasonal H1N1 Hemagglutinin Antibody: Influenza A virus is a major public health threat, killing more than 30,000 people per year in the USA. In early 2009, a novel swine-origin influenza A (H1N1) virus was identified in specimens obtained from patients in Mexico and the United States. The virus spread quickly around the world and on June 11, 2009, the World Health Organization declared it a pandemic. Influenza A virus has one of sixteen possible Hemagglutinin (HA) surface proteins and one of nine possible Neuraminidase (NA) surface proteins. The Hemagglutinin protein facilitates viral attachment while Neuraminidase is involved in viral release. These proteins also elicit immune responses that prevent infection or independently reduce viral replication. The genetic make-up of this swine flu virus is unlike any other: it is an H1N1 strain that combines a triple assortment first identified in 1998 including human, swine, and avian influenza with two new pig H3N2 virus genes from Eurasia, themselves of recent human origin. The distinct antigenic properties of the new swine virus compared with seasonal influenza A (H1N1) virus suggest that human immunity against new swine influenza virus is limited, although the age distribution of reported cases suggests some degree of protection in older age groups.
Background Reference 1:
Thompson WW, Shay DK, Weintraub, et al. Mortality associated with influenza and respiratory syncytial virus in the United States. JAMA 2003; 289:179-186.
Background Reference 2:
Novel Swine-Origin Influenza A (H1N1) Virus Investigation Team, Dawood FS, Jain S, et al. Emergence of a novel swine-origin influenza A (H1N1) virus in humans. N. Engl. J. Med. 2009; 360:2605-15.
Background Reference 3:
Butler D. Swine flu goes global. Nature 2009; 458:1082-3.
Background Reference 4:
Morens DM, Taubenberger JK, and Fauci AS. The Persistent Legacy of the 1918 Influenza Virus. N. Engl. J. Med. 2009; Jun 29.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5235

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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